Examination of the Stem Cell Differentiation During Embryogenesis Using Monoclonal Antibodies

本研究的目的是利用单抗研究干细胞在胚胎发育过程中的增殖和分化行为。这项研究将利用蛇床子门的幼虫形式--扁平水蚤的间质细胞系统，努力在细胞水平上阐明在胚胎发育过程中调节干细胞承诺和分化的控制因素。将鉴定针对扁平间质细胞、它们的分化中间体和它们的后代的单抗。汉斯·博德博士有一个专为成年九头蛇细胞建立的单克隆库，其中一些标记了九头蛇的所有间质细胞，另一些标记了特定的I细胞谱系。博德博士同意提供这些单克隆体，以确定它们是否可以作为胚胎细胞的细胞类型特异性标记物。如果九头蛇单克隆体不起作用，马丁博士将生成一个行星单克隆体库。关于分化细胞的空间模式是如何在胚胎发育过程中建立并最终维持在成体状态的，人们知之甚少。发育生物学中的一个重要问题是细胞如何被限制在特定的分化途径上。这项研究是一个持续计划的一部分，该计划旨在检查胚胎发育期间干细胞承诺和分化所涉及的过程。

The aim of this research is to characterize the proliferation and differentiation behaviour of stem cells during embryogenesis using monoclonal antibodies. The research will utilize the interstitial cell system of hydrozoan planulae, the larval form of the phylum Cnidaria, in efforts to elucidate at the cellular level controlling factors which regulate stem cell commitment and differentiation during embryogenesis. Moloclonal antibodies specific for planular interstitial cells, their differentiative intermediates and their progeny will be identified. Dr. Hans Bode has a library of monoclonals specific for adult hydra cells, some of which label all the interstitial cells of hydra while others label specific I-cell lineages. Dr. Bode has agreed to supply these monoclonals to determine if they can serve as cell type specific markers for embryonic cells. If hydra monoclonals do not work, Dr. Martin will generate a library of planular monoclonals. Very little is known about how the spatial pattern of differentiated cells is established during embryogenesis and consequently maintained in the adult state. An important question in developmental biology is how cells become restricted to a specific pathway of differentiation. This research is part of a continuing plan to examine the processes involved in the commitment and differentiation of stem cells during embryogenesis.