Examination of the Stem Cell Differentiation During Embryogenesis Using Monoclonal Antibodies

使用单克隆抗体检查胚胎发生过程中的干细胞分化

• 批准号: 8711245
• 负责人: Vicki Martin
• 金额: $ 5.4万
• 依托单位: University of Notre Dame
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-01 至 1990-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8711245&HistoricalAwards=false
• 关键词: Examination; Stem; Cell; Differentiation; During

The aim of this research is to characterize the proliferation and differentiation behaviour of stem cells during embryogenesis using monoclonal antibodies. The research will utilize the interstitial cell system of hydrozoan planulae, the larval form of the phylum Cnidaria, in efforts to elucidate at the cellular level controlling factors which regulate stem cell commitment and differentiation during embryogenesis. Moloclonal antibodies specific for planular interstitial cells, their differentiative intermediates and their progeny will be identified. Dr. Hans Bode has a library of monoclonals specific for adult hydra cells, some of which label all the interstitial cells of hydra while others label specific I-cell lineages. Dr. Bode has agreed to supply these monoclonals to determine if they can serve as cell type specific markers for embryonic cells. If hydra monoclonals do not work, Dr. Martin will generate a library of planular monoclonals. Very little is known about how the spatial pattern of differentiated cells is established during embryogenesis and consequently maintained in the adult state. An important question in developmental biology is how cells become restricted to a specific pathway of differentiation. This research is part of a continuing plan to examine the processes involved in the commitment and differentiation of stem cells during embryogenesis.

本研究的目的是利用单克隆抗体表征胚胎发生过程中干细胞的增殖和分化行为。本研究将利用刺胞门的幼虫——浮藻的间质细胞系统，在细胞水平上阐明胚胎发生过程中调节干细胞承诺和分化的控制因素。将鉴定针对扁平间质细胞、其分化中间体及其后代的单克隆抗体。Hans Bode博士有一个针对成年水螅细胞的单克隆库，其中一些标记了水螅的所有间质细胞，而另一些标记了特定的i细胞系。博德博士同意提供这些单克隆基因以确定它们是否可以作为胚胎细胞的细胞类型特异性标记。如果水螅单克隆不起作用，马丁博士将生成一个planular单克隆库。在胚胎发生过程中，分化细胞的空间模式是如何建立的，并因此在成体状态下维持的，我们所知甚少。发育生物学中的一个重要问题是细胞如何局限于特定的分化途径。这项研究是一项持续计划的一部分，旨在研究胚胎发生过程中干细胞的承诺和分化过程。