Examination of the Stem Cell Differentiation During Embryogenesis Using Monoclonal Antibodies

这项研究的目的是描述增殖和 胚胎发生过程中干细胞的分化行为 克隆抗体 这项研究将利用间质细胞 系统的水螅planulae，幼虫形式的刺胞动物门，在 在细胞水平上阐明控制因素的努力， 在胚胎发生过程中调节干细胞定型和分化。 特异于扁平间质细胞的分子克隆抗体，其 将鉴定分化的中间体和它们的后代。 博士 Hans Bode有一个针对成年水螅细胞的单克隆抗体库， 其中一些标记水螅的所有间质细胞， 标记特异性I细胞谱系。 博德博士同意提供这些 单克隆抗体，以确定它们是否可以作为细胞类型特异性标志物 胚胎细胞。 如果九头蛇单克隆抗体不起作用， 生成平面单克隆抗体库。 我们对不同物种的空间分布模式知之甚少。 细胞在胚胎发生过程中建立，并因此维持在 成人国家。 发育生物学中的一个重要问题是 细胞被限制在特定的分化途径中。 这 研究是一项持续计划的一部分， 干细胞的定型和分化 胚胎发生

The aim of this research is to characterize the proliferation and differentiation behaviour of stem cells during embryogenesis using monoclonal antibodies. The research will utilize the interstitial cell system of hydrozoan planulae, the larval form of the phylum Cnidaria, in efforts to elucidate at the cellular level controlling factors which regulate stem cell commitment and differentiation during embryogenesis. Moloclonal antibodies specific for planular interstitial cells, their differentiative intermediates and their progeny will be identified. Dr. Hans Bode has a library of monoclonals specific for adult hydra cells, some of which label all the interstitial cells of hydra while others label specific I-cell lineages. Dr. Bode has agreed to supply these monoclonals to determine if they can serve as cell type specific markers for embryonic cells. If hydra monoclonals do not work, Dr. Martin will generate a library of planular monoclonals. Very little is known about how the spatial pattern of differentiated cells is established during embryogenesis and consequently maintained in the adult state. An important question in developmental biology is how cells become restricted to a specific pathway of differentiation. This research is part of a continuing plan to examine the processes involved in the commitment and differentiation of stem cells during embryogenesis.