Workshop in Bioimaging for HBC Faculty and Undergraduates: to be held June, 2006 at Appalachian State University, Boone, NC

该奖项为为期三周的研讨会提供支持，该研讨会为PI机构和附近两所历史悠久的黑人大学的教师和本科生提供现代激光扫描共聚焦成像的实践。 参加者将掌握新的研究技能，包括从厚荧光标记标本中成像薄光学切片;使用堆叠光学切片进行定量三维测量;使用多荧光成像和延时记录;使用对局部敏感的荧光指示剂（细胞内）游离离子浓度、膜电位、酶活性和/或其它生理化学参数;以及定量共定位分析。 这些和其他显微技术对现代细胞生物学研究至关重要。 该讲习班预计将加强三所大学的教师与由PI指导的阿普拉西安州立大学显微镜中心的工作人员之间的联系，并丰富参与教师提供的课程内容。

This award provides support for a three week workshop offering hands-on in modern laser scanning confocal imaging to faculty and undergraduates from the PI's institution and from two nearby historically black universities. Participants will be expected to master new research skills, including the imaging of thin optical sections from within thick fluorescence-labeled specimens; the use of stacked optical sections in making quantitative three-dimensional measurements; the use of multifluorescence imaging and time-lapse recordings; the use of fluorescent indicators sensitive to local (intracellular) free ion concentration, membrane potential, enzymatic activity and/or other physiochemical parameters; and to quantitative colocalization analyses. These and other microscopic techniques are critical to modern cell biology research. The workshop is expected to strengthen links between faculty in the three universities and the staff of the Applacian State University Microscopy Center, which is directed by the PI, and to enrich the content of coursework offered by the participating faculty.