The T cell genome in 3D: linking chromatin structure to cellular function

3D 中的 T 细胞基因组：将染色质结构与细胞功能联系起来

• 批准号: DP200102405
• 负责人: Prof Stephen Turner
• 金额: $ 49.52万
• 依托单位: Monash University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2020
• 资助国家: 澳大利亚
• 起止时间: 2020-06-01 至 2024-06-01
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP200102405
• 关键词: cell; genome; 3D; linking; chromatin

Adaptive immune cell activation results in the acquisition and long term maintenance of specific cellular function that enables efficient immune control of infections. Using advanced cellular and genomic approaches, combined with high-resolution microscopy and cutting edge computational biology, this proposal aims to address major gaps in our knowledge about how alterations in genomic 3D architecture and targeted biochemical modifications impact cell specific gene nuclear positioning and how this regulates changes in gene expression associated with immune cell activation. An outcome will be identification of novel molecular mechanisms that will have broad applicability across cellular biology, and provide novel targets for drug development.

适应性免疫细胞激活导致获得和长期维持特异性细胞功能，从而能够有效地免疫控制感染。使用先进的细胞和基因组方法，结合高分辨率显微镜和尖端的计算生物学，该提案旨在解决我们知识中的主要空白，即基因组3D结构和靶向生化修饰的改变如何影响细胞特异性基因核定位，以及这如何调节与免疫细胞激活相关的基因表达变化。结果将是鉴定新的分子机制，这些机制将在细胞生物学中具有广泛的适用性，并为药物开发提供新的靶点。