U.S.-Hungary Biochemical Research on Structurally Related Serine Proteases

加州大学旧金山分校的William Rutter博士和布达佩斯Eotvos大学的Laszlo Graf博士开展的这项美国-匈牙利合作研究项目的主要目标是研究丝氨酸蛋白酶(酶)的催化机制，从而解决与这些酶的蛋白质结构和作用相关的未解决的问题。其目的是辨别丝氨酸蛋白酶的基本分子特征，以便了解底物专一性的结构基础的关键方面。结果为定点突变在蛋白质分子工程中的应用提供了一个很好的测试案例。这个生物化学项目通过使美国和东欧的顶尖专家能够在具有强烈共同兴趣和能力的领域结合互补的人才、汇集资源和共享研究设施，实现了促进科学知识发展的计划目标。

The primary objective of this U.S.-Hungary cooperative research project between Dr. William Rutter of the University of California, San Francisco , and Dr. Laszlo Graf of Eotvos University, Budapest, is to study the catalytic mechanisms of serine proteases (enzymes) and thereby address unsolved issues related to protein structure and action of these enzymes. The intent is to discern the fundamental molecular features of serine proteases in order to understand key aspects of the structural basis of substrate specificity. Results should yield a good test case for the application of site-directed mutagenesis in protein molecule engineering. This project in biochemistry fulfills the program objective of advancing scientific knowledge by enabling leading experts in the United States and Eastern Europe to combine complementary talents, pool resources and share research facilities in areas of strong mutual interests and competence.