Development of polymer-based flow cytometry reagents

聚合物流式细胞术试剂的开发

• 批准号: 501147-2016
• 负责人: Seferos, Dwight
• 金额: $ 3.64万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2016
• 资助国家: 加拿大
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=608491
• 关键词: Development; polymer; based; flow; cytometry

Flow cytometry is a powerful technique used in drug discovery, immunology, oncology, proteomics, genomics, and disease control. The global flow cytometry market has been projected to increase from $4.1 billion in 2015 to over $6.3 billion by 2020, driven by an increased demand for high throughput screening techniques in proteomics, genomics, and stem cell research, as well as the rising prevalence of diseases such as cancer and HIV. In this collaborative project between the University of Toronto and BD Canada (BD), we will develop new polymer-based fluorophores for enhanced multicolour flow cytometry. Working in partnership with BD, the Seferos lab will design and synthesize novel fluorescent polymers incorporating highly emissive chromophores located either within the polymer backbone (Project stream 1), or attached as pendant groups (Project stream 2).

流式细胞术是一种强大的技术，用于药物发现、免疫学、肿瘤学、蛋白质组学、基因组学和疾病控制。全球流式细胞仪市场预计将从2015年的41亿美元增加到2020年的63亿美元，这是由于蛋白质组学、基因组学和干细胞研究中对高通量筛查技术的需求增加，以及癌症和艾滋病毒等疾病发病率的上升。在多伦多大学和BD Canada(BD)的这个合作项目中，我们将开发新的基于聚合物的荧光团，用于增强多色流式细胞术。Seferos实验室将与BD合作，设计和合成新型荧光聚合物，其中包含位于聚合物主干中的高发射性发色团(项目流1)，或作为侧基连接的荧光聚合物(项目流2)。