Acquistion of a Zeiss Axiophot Photomicroscope

这是一项申请资金，以获得一台荧光显微镜，该显微镜将主要由两个研究小组使用。其中一个小组正在研究人类上皮细胞相互识别和粘连的机制。这一过程似乎是由一个完整的膜蛋白介导的，该蛋白已被纯化，并产生了针对该蛋白的抗体。第二个小组正在研究涉及寄生蠕虫有氧代谢和厌氧代谢转换的蛋白质。线粒体蛋白已经被提纯，抗体也在这里产生。这两项研究中的抗体都将被荧光标记，然后作为探针，使用所要求的仪器定位细胞结构中的感兴趣蛋白质。

This is a request for funds to obtain a fluorescence microscope that will be used primarily by two research groups. One group is investigating the mechanism by which human epithelial cells recognize and adhere to each other. The process seems to be mediated by an integral membrane protein which has been purified and against which antibodies have been produced. The second group is investigating proteins involved in the shift between aerobic and anaerobic metabolism in parasitic helminths. Mitochondrial proteins have been purified and antibodies generated here also. The antibodies in both studies will be fluorescently labelled and then used as probes to localize the proteins of interest in the cellular structures, using the requested instrumentation.