Research Initiation: Fundamental Studies of Receptor- Mediated Cell Adhesion Using Two Simple Adhesion Assays

研究启动奖：利用两种简单的粘附试验对受体介导的细胞粘附进行基础研究本建议的重点是使用理论和实验的结合，以进一步我们对细胞粘附的定量知识。这将通过对两种细胞粘附性分析的深入研究来完成：一种是流动条件下的平行板粘附分析，另一种是离心分析。前者是一项重要的测定，它表明了在血管系统和许多生物技术设备中发现的控制粘附类型的因素；后者是生物科学家用来定量黏附性的诊断分析，但其解释和正确实施尚未得到证实。这里提出的系统研究应该会产生重要的结果，并测试关于细胞如何进行粘接接触的假设。它应该为科学家和工程师提供有价值的信息，他们的目标是在疾病状态下操纵细胞行为，或设计和实施涉及粘合剂相互作用的生物技术设备。

RESEARCH INITIATION AWARD: FUNDAMENTAL STUDIES OF RECEPTOR-MEDIATED CELL ADHESION USING TWO SIMPLE ADHESION ASSAYS ABSTRACT The focus of this proposal is to use a combination of theory and experiment to further our quantitative knowledge about cell adhesion. This is to be done through an intensive study of two assays for cell adhesiveness: a parallel plate assay for adhesion under conditions of flow, and a centrifugal assay. The former is an important assay which indicates the factors which control the type of adhesiveness found in the vasculature, and in many biotechnological devices; the latter is a diagnostic assay used by biological scientists to quantitate adhesiveness, but whose interpretation and proper implementation have yet to be demonstrated. The systematic studies proposed here should yield important results and test hypotheses about how cells make adhesive contacts. It should provide valuable information for scientists and engineers whose aims are to manipulate cell behavior in disease states, or to design and implement biotechnological devices which involve adhesive interactions.