Research Initiation: Fundamental Studies of Receptor- Mediated Cell Adhesion Using Two Simple Adhesion Assays

研究启动奖：使用两种简单的黏附试验研究受体介导的细胞黏附的基础研究摘要这一建议的重点是将理论和实践相结合实验以加深我们对细胞黏附的定量知识。这将通过对两种细胞黏附试验的深入研究来完成：流动条件下的平行板黏附试验和离心法。前者是一种重要的检测方法，它表明了控制在血管系统和许多生物技术设备中发现的粘附性类型的因素；后者是生物科学家用来定量粘附性的一种诊断方法，但其解释和适当的实施尚未得到证实。这里提出的系统研究应该会产生重要的结果，并测试关于细胞如何进行粘连接触的假设。它应该为科学家和工程师提供有价值的信息，他们的目标是操纵疾病状态下的细胞行为，或者设计和实施涉及粘连相互作用的生物技术设备。

RESEARCH INITIATION AWARD: FUNDAMENTAL STUDIES OF RECEPTOR-MEDIATED CELL ADHESION USING TWO SIMPLE ADHESION ASSAYS ABSTRACT The focus of this proposal is to use a combination of theory and experiment to further our quantitative knowledge about cell adhesion. This is to be done through an intensive study of two assays for cell adhesiveness: a parallel plate assay for adhesion under conditions of flow, and a centrifugal assay. The former is an important assay which indicates the factors which control the type of adhesiveness found in the vasculature, and in many biotechnological devices; the latter is a diagnostic assay used by biological scientists to quantitate adhesiveness, but whose interpretation and proper implementation have yet to be demonstrated. The systematic studies proposed here should yield important results and test hypotheses about how cells make adhesive contacts. It should provide valuable information for scientists and engineers whose aims are to manipulate cell behavior in disease states, or to design and implement biotechnological devices which involve adhesive interactions.