Rat Basophilic Leukemia Cells: A Model System for Cell Adhesion and Separation

大鼠嗜碱性白血病细胞：细胞粘附和分离的模型系统

• 批准号: 9522516
• 负责人: Daniel Hammer
• 金额: $ 9.63万
• 依托单位: Cornell University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-03-01 至 1997-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9522516&HistoricalAwards=false
• 关键词: Rat; Basophilic; Leukemia; Cells; Model

9522516 Hammer This proposal is on research to use rat basophilic leukemia (RBL) cells to study the relationship between chemical recognition and cell adhesion under hydrodynamic flow. Antigen-coated polyacrylamide gel substrates will be synthesized, and the adhesion of murine immunoglobulin E (lgE)-coated RBL cells to these substrates will be measured in a parallel-plate flow chamber. Adhesion will be measured with optical videomicroscopy. Studies which use multiple lgE-antigen pairs with different properties of chemical recognition will be performed. The effect of adhesion on flow rate, antigen and lgE surface densities, and rate and affinity of chemical binding will be measured. Also, the conditions that promote optimal cell detachment, such as applied hydrodynamic force and soluble antigen competitor concentration, will be determined. In addition, and advanced form of flow chamber and a computer simulation method will be developed. ***

9522516锤子这项建议正在研究使用大鼠嗜碱性白血病细胞来研究流体动力流动下化学识别与细胞黏附的关系。将合成包被抗原的聚丙烯酰胺凝胶底物，并在平行平板流动室中测量包被小鼠免疫球蛋白E(LGE)的RBL细胞与这些底物的粘附性。粘附力将用光学视频显微镜测量。将进行使用具有不同化学识别特性的多个LGE-抗原对的研究。将测量粘附性对流速、抗原和LGE表面密度的影响，以及化学结合的速率和亲和力。此外，还将确定促进最佳细胞分离的条件，如施加的流体动力和可溶性抗原竞争对手的浓度。此外，还将开发先进的流室形式和计算机模拟方法。***