Use of Total Internal Reflection Flourescence and MicropipetAspiration to Measure the Detachment of Cells from Ligand- Coated Surfaces
Use of Total Internal Reflection Flourescence and MicropipetAspiration to Measure the Detachment of Cells from Ligand- Coated Surfaces
批准号:
9009506
负责人:
Daniel Hammer
金额:
$6.47万
依托单位:
依托单位国家:
美国
项目类别:
Continuing Grant
财政年份:
1990
资助国家:
美国
项目状态:
已结题
起止时间:
1990-10-01 至 1992-09-30
中文摘要
这个建议是开发仪器来研究细胞脱离表面时细胞的形态和组织。通过微移液器吸出引起剥离;细胞-基质界面的观察将使用全内反射荧光。细胞粘附在生物医学和生物处理问题中起着至关重要的作用,如生物反应器设计、肿瘤细胞转移和炎症反应的触发。所提出的方法将极大地促进这些重要领域的定量、工程化研究。
英文摘要
This proposal is to develop instrumentation to study the morphology and organization of a cell during detachment of the cell from a surface. The detachment will be caused by aspiration through a micropipette; observations of the cell-substrate interface will be made using total internal reflection fluorescence. Cell adhesion plays a crucial role in both biomedical and bioprocessing problems such as bioreactor design, tumor cell metastasis and the triggering of inflammatory responses. The proposed methodology should greatly facilitate quantitative, engineering-oriented research in these important areas.
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会议论文
2020 Bioinspired Materials GRC/GRS
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批准号:2001234
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项目类别:Standard Grant
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资助金额:$2.0万
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财政年份:2020
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负责人:Daniel Hammer
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依托单位:
ST3: Research Collaborative Network on Information-driven Biomaterials
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批准号:1941318
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项目类别:Standard Grant
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资助金额:$10.0万
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财政年份:2019
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负责人:Daniel Hammer
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依托单位:
Responsive Hybrid Oleosin Nanomaterials
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批准号:1609784
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项目类别:Standard Grant
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资助金额:$39.0万
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财政年份:2016
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负责人:Daniel Hammer
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依托单位:
Responsive Vesicles from Recombinant Oleosin
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批准号:1309556
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项目类别:Continuing Grant
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资助金额:$42.0万
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财政年份:2013
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负责人:Daniel Hammer
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依托单位:
Development of Biodiagnostic Devices based on Differential Adhesion Mediated by DNA Hybridization
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批准号:0314265
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项目类别:Continuing Grant
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资助金额:$45.12万
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财政年份:2003
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负责人:Daniel Hammer
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依托单位:
Development of Biodiagnostic Devices Based on Differential Adhesion Under Flow
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批准号:9986384
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项目类别:Continuing Grant
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资助金额:$38.21万
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财政年份:2000
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负责人:Daniel Hammer
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依托单位:
Rat Basophilic Leukemia Cells: A Model System for Cell Adhesion and Separation
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批准号:9522516
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项目类别:Continuing Grant
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资助金额:$9.63万
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财政年份:1996
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负责人:Daniel Hammer
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依托单位:
Rat Basophilic Leukemia Cells: A Model System for Cell Adhesion and Separation
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批准号:9796090
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项目类别:Continuing Grant
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资助金额:$18.14万
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财政年份:1996
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负责人:Daniel Hammer
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依托单位:
Presidential Young Investigators Award
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批准号:8958632
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项目类别:Continuing Grant
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资助金额:$21.2万
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财政年份:1989
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负责人:Daniel Hammer
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依托单位:
Research Initiation: Fundamental Studies of Receptor- Mediated Cell Adhesion Using Two Simple Adhesion Assays
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批准号:8808867
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项目类别:Standard Grant
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资助金额:$6.68万
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财政年份:1988
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负责人:Daniel Hammer
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依托单位:
Engineering Creativity Award: New Approach to the Growth Rate of Cells Under Limiting Conditions
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批准号:8710373
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项目类别:Continuing Grant
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资助金额:$9.0万
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财政年份:1987
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负责人:Daniel Hammer
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依托单位:
国内基金
海外基金
面向SCR脱硝系统的total NOx传感器混合导电界面设计及性能研
究
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批准号:
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项目类别:省市级项目
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资助金额:--
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批准年份:2024
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负责人:
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依托单位: