Use of Total Internal Reflection Flourescence and MicropipetAspiration to Measure the Detachment of Cells from Ligand- Coated Surfaces

这项建议是开发仪器来研究 脱离期间细胞的形态和组织 从表面上分离细胞。 分遣队将在 通过微量移液管抽吸引起;观察 的细胞-基质界面将使用全 内反射荧光 细胞粘附起着 在生物医学和生物加工中的关键作用 生物反应器设计、肿瘤细胞转移等问题 和炎症反应的触发。 的 拟议的方法应大大有助于 定量的、工程导向的研究， 重要领域。

This proposal is to develop instrumentation to study the morphology and organization of a cell during detachment of the cell from a surface. The detachment will be caused by aspiration through a micropipette; observations of the cell-substrate interface will be made using total internal reflection fluorescence. Cell adhesion plays a crucial role in both biomedical and bioprocessing problems such as bioreactor design, tumor cell metastasis and the triggering of inflammatory responses. The proposed methodology should greatly facilitate quantitative, engineering-oriented research in these important areas.