Use of Total Internal Reflection Flourescence and MicropipetAspiration to Measure the Detachment of Cells from Ligand- Coated Surfaces

这个建议是开发仪器来研究细胞脱离表面时细胞的形态和组织。通过微移液器吸出引起剥离；细胞-基质界面的观察将使用全内反射荧光。细胞粘附在生物医学和生物处理问题中起着至关重要的作用，如生物反应器设计、肿瘤细胞转移和炎症反应的触发。所提出的方法将极大地促进这些重要领域的定量、工程化研究。

This proposal is to develop instrumentation to study the morphology and organization of a cell during detachment of the cell from a surface. The detachment will be caused by aspiration through a micropipette; observations of the cell-substrate interface will be made using total internal reflection fluorescence. Cell adhesion plays a crucial role in both biomedical and bioprocessing problems such as bioreactor design, tumor cell metastasis and the triggering of inflammatory responses. The proposed methodology should greatly facilitate quantitative, engineering-oriented research in these important areas.