Use of Total Internal Reflection Flourescence and MicropipetAspiration to Measure the Detachment of Cells from Ligand- Coated Surfaces

使用全内反射荧光和微量移液器抽吸来测量细胞从配体包被表面的脱离

• 批准号: 9009506
• 负责人: Daniel Hammer
• 金额: $ 6.47万
• 依托单位: Cornell University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-10-01 至 1992-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9009506&HistoricalAwards=false
• 关键词: Use; Total; Internal; Reflection; Flourescence

This proposal is to develop instrumentation to study the morphology and organization of a cell during detachment of the cell from a surface. The detachment will be caused by aspiration through a micropipette; observations of the cell-substrate interface will be made using total internal reflection fluorescence. Cell adhesion plays a crucial role in both biomedical and bioprocessing problems such as bioreactor design, tumor cell metastasis and the triggering of inflammatory responses. The proposed methodology should greatly facilitate quantitative, engineering-oriented research in these important areas.

该提案旨在开发仪器来研究细胞从表面分离过程中的形态和组织。 分离是通过微量移液器抽吸引起的；将使用全内反射荧光来观察细胞-基质界面。 细胞粘附在生物医学和生物加工问题中发挥着至关重要的作用，例如生物反应器设计、肿瘤细胞转移和炎症反应的触发。 所提出的方法将极大地促进这些重要领域的定量、面向工程的研究。