Development of Biodiagnostic Devices based on Differential Adhesion Mediated by DNA Hybridization

基于 DNA 杂交介导的差异粘附的生物诊断装置的开发

• 批准号: 0314265
• 负责人: Daniel Hammer
• 金额: $ 45.12万
• 依托单位: University of Pennsylvania
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-15 至 2007-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0314265&HistoricalAwards=false
• 关键词: Development; Biodiagnostic; Devices; based; Differential

The objective of this project is to develop and optimize methods for the detection of unknown DNA strands by the adhesion of micro-sized particles bearing DNA to surfaces bearing complementary strands of DNA. Such techniques would combine DNA hybridization as a high fidelity means of identifying unknown strands, with bead based adhesion, in which the large number of complementary strands leads to a higher signal-to-noise in DNA detection, as well as a sharper dependence on external variables that affect binding, such as temperature, molecular density and flow rate. Furthermore, the use of micro-sized particles would lead to simple optical detection of binding, which would facilitate and broaden the methods for detection. The methods developed here will be useful for the detection of foreign DNA from pathogenic bacteria, and thus be useful for pathogen detection and the national defense against bioterrorism.

这个项目的目标是开发和优化检测未知DNA链的方法，通过将携带DNA的微小颗粒粘附到携带互补DNA链的表面。这些技术将结合DNA杂交作为鉴定未知链的高保真方法，与基于头的粘附，其中大量互补链导致DNA检测中的高信噪比，以及对影响结合的外部变量的更大依赖，如温度，分子密度和流速。此外，使用微型粒子将导致简单的结合光学检测，这将促进和扩大检测方法。该方法可用于病原菌外源DNA的检测，从而为病原菌检测和防御生物恐怖主义提供依据。