Large Scale Zone Electrophoresis with Solute Recycle

自由流区带电泳在加工中的优势 不稳定的大分子是众所周知的。 这项技术很强大， 温和，普遍适用于生物材料， 从低分子量抗生素到整个细胞。 到 然而，迄今为止，不可能实现高分辨率 和高吞吐量。 在一项早期研究中， 在NSF的支持下，建造了一个原型设备， 实验性地测试能够高吞吐量和 优良的纯化;这些实验表明 该过程的数学模型足以用于放大该方法。 设计 原型模型的蛋白质吞吐量等于 这是目前最好的商业设备。 的 目前的拨款是用于测试一个扩大到 吞吐量大两个数量级，大约100 g/hr的分离能力，并对实际分离效果进行了验证。 工业蛋白质混合物。

The advantages of free-flow zone electrophoresis for processing labile macromolecules are well-known. The technique is powerful, gentle, and universally applicable to biomaterials ranging in size from low molecular weight antibiotics to whole cells. To date, however, it has been impossible to achieve high resolution and high throughput simultaneously. In an early study, also supported by NSF, a prototype device was constructed to experimentally test a design capable of both high throughput and excellent purifications; these experiments indicated the mathematical model of the process is adequate for scale-up of the design. The prototype model has a protein throughput equal to that of the best currently available commercial devices. The present grant is for testing of a device scaled-up to a throughput two orders of magnitude greater, on the order of 100 g/hr, and the demonstration of its separating power for realistic industrial protein mixtures.