Preparative High Performance Capillary Electrophoresis

制备型高性能毛细管电泳

• 批准号: 9014793
• 负责人: Cornelius Ivory
• 金额: --
• 依托单位: Washington State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-05-15 至 1992-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9014793&HistoricalAwards=false
• 关键词: Preparative; Performance; Capillary; Electrophoresis

High performance capillary electrophoresis (HPCE) offers important advantages over conventional gel electrophoresis in the characterization of proteins and nucleic acids. It is much faster than the gels and can be fully automated and instrumented with sample injection, detection and collection. In addition, HPCE can separate solutes with a resolution which is one to two orders of magnitude greater than high performance liquid chromatography (HPLC) using sample sizes four orders of magnitude smaller than HPLC. However, single-capillary HPCE can only handle solutes loadings of less than 1 ug. It cannot be used at preparatory scales to provide material for further analysis, clinical research or therapeutic application. Most scientists familiar with this technology believe that, if HPCE could be scaled to preparative levels, it would become an indispensable laboratory tool. Unfortunately, scale-up to preparative HPCE is not simple: a preparative HPCE column must dissipate Joule heat effectively while maintaining the extraordinarily high plate efficiency routinely observed in ug. HPCE. To reach the goal of 1 mg HPCE, a study is proposed which combines both theoretical and experimental analyses of single and multiple capillary HPCE. The objectives of this research are to (1) elucidate the mechanisms of heat transfer, separation and dispersion in single capillaries and to apply this knowledge in the (2) design of a preparative-scale HPCE column.

高效毛细管电泳（HPCE）在蛋白质和核酸的表征中提供了比传统凝胶电泳更重要的优势。它比凝胶快得多，可以完全自动化，并与样品注射，检测和收集仪器。此外，HPCE可以使用比HPLC小四个数量级的样品量，以比高效液相色谱（HPLC）大一到两个数量级的分辨率分离溶质。然而，单毛细管HPCE只能处理小于1 μ g的溶质负载。它不能用于准备规模，为进一步分析，临床研究或治疗应用提供材料。大多数熟悉这项技术的科学家认为，如果HPCE能够扩展到制备水平，它将成为不可或缺的实验室工具。不幸的是，按比例放大到制备型HPCE并不简单：制备型HPCE柱必须有效地耗散焦耳热，同时保持以μ g为单位常规观察到的非常高的板效率。HPCE。为了达到1毫克HPCE的目标，提出了一项研究，结合理论和实验分析的单和多毛细管HPCE。本研究的目的是（1）阐明在单一毛细管中的传热、分离和分散的机理，并将这些知识应用于（2）设计一个大规模的HPCE柱。