A Four-Dimensional (4D) Microchip for Proteomics

用于蛋白质组学的四维 (4D) 微芯片

• 批准号: 0626471
• 负责人: Cornelius Ivory
• 金额: $ 20万
• 依托单位: Washington State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-15 至 2009-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0626471&HistoricalAwards=false
• 关键词: Four; Dimensional; 4D; Microchip; Proteomics

Abstract Proposal Title: A Four Dimensional (4D) Microchip for Proteomics, Proposal Number: CTS-0626471, Principal Investigator: Cornelius F. Ivory, Institution: Washington State University Abstract:The project aims at developing an alternative strategy for designing nD separations platforms that emphasizes parallel operation and self-sharpening separation protocols. The proposal is divided into three tasks, first approximately one dozen 1D separations will be adapted to operate on a PDMS platform. The second task is a demonstration of how to move fractionated peaks from one separation dimension to the next, and the third task will involve design of a 4D microchip. This project is an ambitious leap forward in the capabilities of separation science. Reliable separations of tens of thousands of proteins, without overlap and of sufficiently narrow band width, would be the cornerstone of any proteome study. This is because analyzing eluates with overlapping proteins introduce unacceptable uncertainty (and possibly mislead researchers) in the mass spectra. The coupling of micro-fluidics in a massively parallel scheme with a deep knowledge of chromatographic separations is critical. Though admittedly a complete 4-D system would not be fabricated, the PI wisely chose to explore the most significant challenges to the integration using the most cutting edge chromatographic techniques applied to micro-fluidics. The track record of the team is noteworthy. The combination of microfabrication, surface chemistry and chromographic expertise is ideal for the success of this project. The insight in the chemical/transport problems and the strategy for multi-dimensional integration is highly promising. From a broader impacts perspective, success in developing a massively parallel cascade separation system will be a cornerstone for enabling the cataloging of the human proteome. Such success will be a breakthrough for the development of medical treatments for a vast number of illnesses. The scheme can also be applied to medical diagnostics. Insight gained in cascade separations can be applied to higher volume pharmaceutical processes. The proposal will directly support 2 graduate students. Additionally, 3+ undergraduate students per year will also be in the working group. Additional support for teacher participation via the NSF-SWEET program is expected and undergraduates under the NSF-REU program. Overall, the proposal will provide quality educational opportunities to a wide range of individuals._______________________________________Eric S. Peterson, Program Director (1417) Date

摘要 提案标题：用于蛋白质组学的四维 (4D) 微芯片，提案编号：CTS-0626471，首席研究员：Cornelius F. Ivory，机构：华盛顿州立大学 摘要：该项目旨在开发一种设计 nD 分离平台的替代策略，强调并行操作和自锐化分离方案。该提案分为三个任务，首先将调整大约十几个一维分离以在 PDMS 平台上运行。第二个任务是演示如何将分级峰从一个分离维度移动到下一个分离维度，第三个任务将涉及 4D 微芯片的设计。该项目是分离科学能力的一次雄心勃勃的飞跃。数万种蛋白质的可靠分离、无重叠且带宽足够窄，将是任何蛋白质组研究的基石。这是因为分析具有重叠蛋白质的洗脱物会在质谱中引入不可接受的不确定性（并可能误导研究人员）。大规模并行方案中微流体的耦合与对色谱分离的深入了解至关重要。尽管无法制造完整的 4-D 系统，但 PI 明智地选择使用应用于微流体的最尖端色谱技术来探索集成的最重大挑战。该团队的往绩记录值得注意。微加工、表面化学和色谱专业知识的结合是该项目成功的理想选择。对化学/运输问题的洞察力和多维整合策略非常有前途。从更广泛的影响角度来看，成功开发大规模并行级联分离系统将成为人类蛋白质组编目的基石。这样的成功将成为针对大量疾病的医学治疗方法的突破。该方案还可以应用于医学诊断。在级联分离中获得的见解可以应用于更大容量的制药过程。该提案将直接支持2名研究生。此外，每年3名以上本科生也将加入工作组。预计通过 NSF-SWEET 计划为教师参与提供额外支持，并为 NSF-REU 计划下的本科生提供额外支持。总体而言，该提案将为广大个人提供优质的教育机会。 ____________________________________Eric S. Peterson，项目总监 (1417) 日期