A Four-Dimensional (4D) Microchip for Proteomics

项目名称：一种用于蛋白质组学的四维（4D） Microchip，项目编号：CTS-0626471，项目负责人：Cornelius F. Ivory，机构：华盛顿州立大学摘要：该项目旨在开发一种用于设计nD分离平台的替代策略，该平台强调并行操作和自锐分离协议。该提案分为三个任务，首先，大约12个1D分离将适应PDMS平台上的操作。第二个任务是演示如何将分选峰从一个分离维度移动到下一个，第三个任务将涉及4D微芯片的设计。这个项目是分离科学能力的一次雄心勃勃的飞跃。可靠地分离数以万计的蛋白质，没有重叠和足够窄的频带宽度，将是任何蛋白质组学研究的基石。这是因为分析有重叠蛋白质的洗脱液会在质谱中引入不可接受的不确定性（并可能误导研究人员）。微流体在大规模平行方案中的耦合与色谱分离的深入知识是至关重要的。虽然不可否认，一个完整的4-D系统是不可能制造出来的，但PI明智地选择了利用应用于微流体的最尖端色谱技术来探索集成的最重大挑战。该团队的业绩记录值得注意。微加工，表面化学和色谱专业知识的结合是这个项目成功的理想选择。在化学/运输问题的洞察力和多维整合策略是非常有前途的。从更广泛的影响角度来看，开发大规模平行级联分离系统的成功将成为实现人类蛋白质组编目的基石。这样的成功将是对许多疾病的医学治疗发展的一个突破。该方案也可应用于医学诊断。在级联分离中获得的洞察力可以应用于更高体积的制药过程。该提案将直接支持2名研究生。此外，每年还将有3名以上的本科生加入工作组。期望通过NSF-SWEET项目为教师参与和NSF-REU项目的本科生提供额外的支持。总的来说，该提案将为广泛的个人提供优质的教育机会。_______________________________________ 项目负责人埃里克·s·彼得森(1417)日期

Abstract Proposal Title: A Four Dimensional (4D) Microchip for Proteomics, Proposal Number: CTS-0626471, Principal Investigator: Cornelius F. Ivory, Institution: Washington State University Abstract:The project aims at developing an alternative strategy for designing nD separations platforms that emphasizes parallel operation and self-sharpening separation protocols. The proposal is divided into three tasks, first approximately one dozen 1D separations will be adapted to operate on a PDMS platform. The second task is a demonstration of how to move fractionated peaks from one separation dimension to the next, and the third task will involve design of a 4D microchip. This project is an ambitious leap forward in the capabilities of separation science. Reliable separations of tens of thousands of proteins, without overlap and of sufficiently narrow band width, would be the cornerstone of any proteome study. This is because analyzing eluates with overlapping proteins introduce unacceptable uncertainty (and possibly mislead researchers) in the mass spectra. The coupling of micro-fluidics in a massively parallel scheme with a deep knowledge of chromatographic separations is critical. Though admittedly a complete 4-D system would not be fabricated, the PI wisely chose to explore the most significant challenges to the integration using the most cutting edge chromatographic techniques applied to micro-fluidics. The track record of the team is noteworthy. The combination of microfabrication, surface chemistry and chromographic expertise is ideal for the success of this project. The insight in the chemical/transport problems and the strategy for multi-dimensional integration is highly promising. From a broader impacts perspective, success in developing a massively parallel cascade separation system will be a cornerstone for enabling the cataloging of the human proteome. Such success will be a breakthrough for the development of medical treatments for a vast number of illnesses. The scheme can also be applied to medical diagnostics. Insight gained in cascade separations can be applied to higher volume pharmaceutical processes. The proposal will directly support 2 graduate students. Additionally, 3+ undergraduate students per year will also be in the working group. Additional support for teacher participation via the NSF-SWEET program is expected and undergraduates under the NSF-REU program. Overall, the proposal will provide quality educational opportunities to a wide range of individuals._______________________________________Eric S. Peterson, Program Director (1417) Date