Focusing of Proteins in a Multi-Chamber Electro- Ultrafiltration (MEUF) Column

9417239 Ivory本项目是研究电泳-超滤耦合分离聚电解质。 其基本思想是，由电场引起的电泳传输与由压力流和溶剂的电渗引起的穿过多孔屏障（膜）的对流传输之间的竞争可用于基于其电泳迁移率来分离带电大分子如蛋白质。 已经用一种溶质和一种多孔屏障完成了初步工作。 拟议的研究将调查和开发一个系统与多障碍和多组分的解决方案，与分馏的蛋白质混合物到不同的隔间由两个多孔屏障的目标。 除了这个装置，研究将开发一个模型的电动现象的内部和外部的膜孔。 ***

9417239 Ivory This project is on research to study separation of polyelectrolytes by coupling electrophoresis and ultrafiltration. The basic idea is that competition between electrophoretic transport, caused by an electric field, and convective transport across a porous barrier (a membrane), caused by pressure flow and electroosmosis of solvent, can be used to separate charged macromolecules such as proteins on the basis of their electrophoric mobility. Preliminary work has been done with one solute and one porous barrier. The proposed research would investigate and develop a system with multibarriers and multicomponent solutions, with the objective of fractionating a mixture of proteins into different compartments bounded by two porous barriers. In addition to this apparatus, the research will develop a model of the electrokinetic phenomena inside and outside of the membrane pores. ***