Focusing of Proteins in a Multi-Chamber Electro- Ultrafiltration (MEUF) Column

9417239象牙本课题研究的是耦合电泳和超滤分离聚电解质的方法。其基本思想是，电场引起的电泳传输和溶剂的压力流和电渗透引起的多孔屏障（膜）上的对流传输之间的竞争，可以用来根据蛋白质等带电大分子的电迁移率分离带电大分子。用一个溶质和一个多孔势垒做了初步的工作。拟议的研究将调查和开发一个具有多屏障和多组分溶液的系统，其目标是将蛋白质混合物分离到由两个多孔屏障隔开的不同隔间中。除了这个装置外，研究还将开发一个膜孔内外的电动现象模型。* * *

9417239 Ivory This project is on research to study separation of polyelectrolytes by coupling electrophoresis and ultrafiltration. The basic idea is that competition between electrophoretic transport, caused by an electric field, and convective transport across a porous barrier (a membrane), caused by pressure flow and electroosmosis of solvent, can be used to separate charged macromolecules such as proteins on the basis of their electrophoric mobility. Preliminary work has been done with one solute and one porous barrier. The proposed research would investigate and develop a system with multibarriers and multicomponent solutions, with the objective of fractionating a mixture of proteins into different compartments bounded by two porous barriers. In addition to this apparatus, the research will develop a model of the electrokinetic phenomena inside and outside of the membrane pores. ***