U.S.-Japan Cooperative Research: Fundamental Study of Receptor Ion-Channel Sensors

美日合作研究：受体离子通道传感器的基础研究

• 批准号: 8815739
• 负责人: Theodore Kuwana
• 金额: --
• 依托单位: University of Kansas Main Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-04-01 至 1993-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8815739&HistoricalAwards=false
• 关键词: U.S.; Japan; Cooperative; Research; Fundamental

This award will enable Prof. Theodore Kuwana and colleagues at the University of Kansas to collaborate with Prof. Yoshio Umezawa of Hokkaido University over a period of two years. They will attempt to develop ultrasensitive and selective sensors for the detection of biological substances, including neurotoxins. These sensors will be based on the use of a protein-based receptor, which acts as a gate for a membrane ionchannel. In particular, this research will focus on the glutamate binding protein, which is a constituent in the most widespread excitatory chemical transmitter system in the central nervous system, and is a candidate for a suitable protein-based receptor. The purpose of this research is to enhance the understanding of the glutamate binding protein, and the program includes the isolation and purification of GBP receptor ion channels, reconstruction of these channels in various synthetic membranes, structural and functional characterization, and determination of their genetic properties. The work brings together acknowledged experts on protein synthesis and analysis from the two countries, and could have important implications in the development of selective chemical probes.

这一奖项将使堪萨斯大学的Theodore Kuwana教授及其同事能够在两年内与北海道大学的Yoshio Umezawa教授合作。他们将尝试开发超灵敏和选择性的传感器来检测生物物质，包括神经毒素。这些传感器将基于蛋白质受体的使用，该受体充当膜离子通道的闸门。特别是，这项研究将集中在谷氨酸结合蛋白，它是中枢神经系统中最广泛的兴奋性化学递质系统的组成部分，也是合适的基于蛋白质的受体的候选者。本研究的目的是加深对谷氨酸结合蛋白的了解，包括分离和纯化GBP受体离子通道，在各种合成的膜上重建这些通道，结构和功能表征，以及它们的遗传特性的测定。这项工作汇集了两国公认的蛋白质合成和分析专家，并可能对选择性化学探针的开发产生重要影响。