Antarctic Marine Yeasts: Preservation and Distribution of aLiving Collection

该项目将保存并提供通过美国国家科学基金会资助的南极、南太平洋和印度洋微生物生态学获得的活酵母。这些研究是在1960年代rv Anton Bruun， USNS Eltanin和USCGC破冰船Eastwind的巡航期间进行的。作为研究的一部分，3000多株酵母菌被分离出来并进行了相当详细的研究。这些藏品代表了从极端和相对难以接近的环境中分离出来的独特生物基因库。其中一些菌株是通过国际收集提供的，在系统学、分子生物学、生物化学、酶学和生物技术等领域产生了相当大的研究。在研究完成后，将收集的菌种冷冻干燥，以使菌种在最少的维护下存活。在储存期间，冻干瓶的密封已经恶化，收集的生存能力处于危险之中。此外，由于应变数据在原始的野外和实验室数据书中，感兴趣的调查人员很难使用这些数据集。该奖项将使整个收集和数据随时可用。收集的样品将在长期储存瓶中进行再干燥，并将应变信息放入计算机数据库中。收集和数据将被纳入美国农业部农业研究服务文化收藏，该收藏是世界上最大的微生物收藏之一。将为农业、工业和学术研究提供的菌株将得到保留和分配。

This project will preserve and make available a collection of living yeasts obtained through National Science Foundation funding of microbial ecology in the Antarctic, southern Pacific and Indian Oceans. These studies were conducted during cruises of the R.V. Anton Bruun, USNS Eltanin and the USCGC Icebreaker Eastwind during the l960's. As part of the research, more than 3000 strains of yeasts were isolated and studied in considerable detail. The collection represents a unique genetic pool of organisms isolated from an extreme and relatively inaccessible environment. Some of the strains from the collection, which were made available through international collections, generated considerable research in the areas of systematics, molecular biology, biochemistry, enzymology and biotechnology. At the completion of the research, the collection was freeze- dried to keep the strains alive with minimal maintenance. During storage, the seals of the lyophilization vials have deteriorated and the viability of the collection is in jeopardy. In addition, it is difficult for interested investigators to use the collection because the strain data is in the original field and laboratory data books. This award will to make the entire collection and data readily available. The collection will be relyophilized in long term storage vials and the strain information placed in a computer data bank. The collection and data will be included in the Agricultural Research Service Culture Collection, U.S. Department of Agriculture, which maintains one of the world's largest microbial collections. Strains to be made available for agricultural, industrial and academic research, will be maintained and dispensed.