Antarctic Marine Yeasts: Preservation and Distribution of aLiving Collection

该项目将保存并提供一系列 通过国家科学基金会资助获得的活酵母 南极、南太平洋和印度的微生物生态学 海洋. 这些研究是在R. V.巡航期间进行的。 Anton Bruun，USNS Eltanin和USCGC破冰船Eastwind 1960年代 作为研究的一部分，超过3000种菌株 分离酵母并进行了相当详细的研究。 的 收集代表了一个独特的生物遗传库分离 从一个极端的和相对难以接近的环境。 一些 从收集的菌株，这是通过提供 国际收藏，产生了相当大的研究， 系统学、分子生物学、生物化学、酶学领域 和生物技术。 在研究结束时，收集被冻结- 干燥以保持菌株存活，并进行最少的维护。 期间 储存时，冻干瓶的密封已损坏 收藏品的可行性岌岌可危 此外，本发明还提供了一种方法， 感兴趣的研究人员很难使用这些收藏品， 因为应变数据在原始现场和实验室中 数据书 这个奖项将使整个收集和 数据随时可用。 收藏品将在 长期储存瓶和应变信息放在一个 计算机数据库 收集和数据将包括在 美国农业研究服务培养物保藏中心 农业部，它保持着世界上最大的 最大的微生物收藏 菌株可用于 农业，工业和学术研究，将保持 并分发。

This project will preserve and make available a collection of living yeasts obtained through National Science Foundation funding of microbial ecology in the Antarctic, southern Pacific and Indian Oceans. These studies were conducted during cruises of the R.V. Anton Bruun, USNS Eltanin and the USCGC Icebreaker Eastwind during the l960's. As part of the research, more than 3000 strains of yeasts were isolated and studied in considerable detail. The collection represents a unique genetic pool of organisms isolated from an extreme and relatively inaccessible environment. Some of the strains from the collection, which were made available through international collections, generated considerable research in the areas of systematics, molecular biology, biochemistry, enzymology and biotechnology. At the completion of the research, the collection was freeze- dried to keep the strains alive with minimal maintenance. During storage, the seals of the lyophilization vials have deteriorated and the viability of the collection is in jeopardy. In addition, it is difficult for interested investigators to use the collection because the strain data is in the original field and laboratory data books. This award will to make the entire collection and data readily available. The collection will be relyophilized in long term storage vials and the strain information placed in a computer data bank. The collection and data will be included in the Agricultural Research Service Culture Collection, U.S. Department of Agriculture, which maintains one of the world's largest microbial collections. Strains to be made available for agricultural, industrial and academic research, will be maintained and dispensed.