Antarctic Marine Yeasts: Preservation and Distribution of aLiving Collection

该项目将保存和提供通过国家科学基金会资助的南极、南太平洋和印度洋微生物生态学获得的一批活酵母。这些研究是在1960年S年间安东·布鲁恩号、埃尔塔宁号和东风号巡航期间进行的。作为研究的一部分，分离和研究了3000多株酵母菌。这个集合代表了一个从极端和相对难以进入的环境中分离出来的独特的有机体基因库。收集的一些菌株是通过国际收藏提供的，在系统学、分子生物学、生物化学、酶学和生物技术领域产生了大量研究。在研究完成时，收集的样品被冷冻干燥，以最少的维护保持菌株的存活。在储存过程中，冻干瓶的封口已经变质，收藏品的生存能力处于危险之中。此外，感兴趣的调查人员很难使用收集的菌株数据，因为菌株数据在原始的现场和实验室数据手册中。这一奖项将使整个收集和数据变得容易获得。收集的样品将长期储存在小瓶中重新冷冻，并将菌株信息存储在计算机数据库中。这些收集和数据将被包括在美国农业部农业研究服务培养库中，该库维护着世界上最大的微生物馆藏之一。供农业、工业和学术研究使用的菌株将得到维护和分配。

This project will preserve and make available a collection of living yeasts obtained through National Science Foundation funding of microbial ecology in the Antarctic, southern Pacific and Indian Oceans. These studies were conducted during cruises of the R.V. Anton Bruun, USNS Eltanin and the USCGC Icebreaker Eastwind during the l960's. As part of the research, more than 3000 strains of yeasts were isolated and studied in considerable detail. The collection represents a unique genetic pool of organisms isolated from an extreme and relatively inaccessible environment. Some of the strains from the collection, which were made available through international collections, generated considerable research in the areas of systematics, molecular biology, biochemistry, enzymology and biotechnology. At the completion of the research, the collection was freeze- dried to keep the strains alive with minimal maintenance. During storage, the seals of the lyophilization vials have deteriorated and the viability of the collection is in jeopardy. In addition, it is difficult for interested investigators to use the collection because the strain data is in the original field and laboratory data books. This award will to make the entire collection and data readily available. The collection will be relyophilized in long term storage vials and the strain information placed in a computer data bank. The collection and data will be included in the Agricultural Research Service Culture Collection, U.S. Department of Agriculture, which maintains one of the world's largest microbial collections. Strains to be made available for agricultural, industrial and academic research, will be maintained and dispensed.