Ribosomal DNA Sequences in Marine Yeasts: A Model for Identification and Quantification of Marine Eukaryotes

9504105 Fall利用分子技术快速而准确地确定群落结构，这项研究将确定两类截然不同的微型真菌的真菌生物多样性和种群生物量：担子菌酵母和卵菌属盐生真菌。这两个群体在碎屑食物网中都扮演着重要的角色。研究计划将包括实验室和实地研究。实验室研究将完成已知物种的数据库，作为确定野外群落结构的基础。将开发新的程序，初步强调使用激光检测的红外标记引物进行定量聚合酶链式反应(QPCR)。实地研究将集中在珊瑚礁和红树林栖息地。利用经典微生物技术和分子方法的结合，将确定已知和未知可培养真菌物种的群落结构和相对丰度。这些物种的身份将通过自动DNA序列分析和与数据库的核苷酸比对来确定。将定位特定物种的区域并开发引物，以测试聚合酶链式反应技术在估计群落结构方面的准确性和敏感性。通过使用聚合酶链式反应和定量聚合酶链式反应，将估计不可培养物种的出现和种群密度。本研究开发的技术可应用于其他微型或大型真核生物群落的种群分析。

9504105 Fell Using molecular techniques for rapid and accurate determination of community structure, this research will determine fungal biodiversity and population biomass of two distinctly different groups of micro-fungi: the basidiomycetous yeasts and the oomycetous genus Halophythophora. Both groups have important roles in detrital based food webs. The research program will include laboratory and field studies. Laboratory studies will complete the data bank of know species as a basis for determining community structure in the field. New procedures will be developed with preliminary emphasis on quantitative PCR (QPCR) using laser detected infrared labeled primers. Field research will center on reef and mangrove habitats. Using a combination of classical microbial techniques and molecular methods, the community structure and relative abundance of known and unknown culturable fungi species will be determined. The identity of these species will be ascertained by automated DNA sequence analysis and nucleotide alignment with the data bank. Species-specific regions will be located and primers developed to test the accuracy and sensitivity of PCR techniques in estimating community structure. Through the use of PCR and QPCR, the occurrence of unculturable species and population densities will be estimated. The techniques developed in this research can be applied to population analyses of other micro- or macro-eukaryote communities.