Ribosomal DNA Sequences in Marine Yeasts: A Model for Identification and Quantification of Marine Eukaryotes

小行星9504105 利用分子生物学技术，快速，准确地确定社区结构，本研究将确定真菌的生物多样性和人口生物量的两个明显不同的群体的微型真菌：担子菌酵母和卵菌属Halophythophora。 这两个群体在以碎屑为基础的食物网中都发挥着重要作用。 研究计划将包括实验室和实地研究。 实验室研究将完成已知物种的数据库，作为确定实地群落结构的基础。 新的程序将与 初步强调了激光检测红外标记引物的定量PCR（QPCR）。 实地研究将集中在珊瑚礁和红树林栖息地。 使用经典的微生物技术和分子方法的组合，已知和未知的可培养真菌物种的群落结构和相对丰度将被确定。 这些物种的身份将通过自动DNA序列分析和与数据库的核苷酸比对来确定。 将确定物种特异性区域，并开发引物，以测试PCR技术在估计群落结构方面的准确性和灵敏度。 通过使用PCR和QPCR，将估计不可养殖物种的发生率和种群密度。 本研究中开发的技术可应用于其他微型或大型真核生物群落的种群分析。

9504105 Fell Using molecular techniques for rapid and accurate determination of community structure, this research will determine fungal biodiversity and population biomass of two distinctly different groups of micro-fungi: the basidiomycetous yeasts and the oomycetous genus Halophythophora. Both groups have important roles in detrital based food webs. The research program will include laboratory and field studies. Laboratory studies will complete the data bank of know species as a basis for determining community structure in the field. New procedures will be developed with preliminary emphasis on quantitative PCR (QPCR) using laser detected infrared labeled primers. Field research will center on reef and mangrove habitats. Using a combination of classical microbial techniques and molecular methods, the community structure and relative abundance of known and unknown culturable fungi species will be determined. The identity of these species will be ascertained by automated DNA sequence analysis and nucleotide alignment with the data bank. Species-specific regions will be located and primers developed to test the accuracy and sensitivity of PCR techniques in estimating community structure. Through the use of PCR and QPCR, the occurrence of unculturable species and population densities will be estimated. The techniques developed in this research can be applied to population analyses of other micro- or macro-eukaryote communities.