Ribosomal RNA Sequences in Marine Yeasts: A Model for Identification and Quantification of Marine Eukaryotes

迫切需要鉴定和量化许多类型的小型海洋真核生物的技术。由于培养和鉴定海洋微真核生物的困难，目前对种群动态的了解仅限于微生物群落的一小部分。这些社区分析中的问题可以通过分子生物学的进步而开发的工具来解决。本研究的目的是开发用于酵母种群快速分析的非放射性探针。将开发三种类型的探针来确定真核生物群落中酵母种群的相对丰度：通用真核生物探针，通用酵母探针和物种特异性探针。费尔博士将对化学发光、荧光和生物素标记探针进行实验。这些探针将在实验室和现场进行测试。

There is an urgent need for techniques to identify and quantify many types of small marine eukaryotes. Current knowledge of population dynamics is limited to a small percentage of the microbial community, due to the difficulties involved with culturing and identifying marine microeukaryotes. These problems in community analysis can be resolved with tools that are being developed through advances in molecular biology. Objectives of the investigation are to develop non- radioactive probes for rapid analyses of populations of yeasts. Three types of probes will be developed to determine the relative abundance of yeast populations within the eukaryotic community: a universal eukaryote probe, a universal yeast probe and species specific probes. Dr. Fell will experiment with chemiluminescent, fluorescent and biotin labeled probes. These probes will be tested in the laboratory and in the field.