Collaborative Research: Developing Gene-Based Remote Detection

Goodwin0332793ABSTRACT该奖项提供资金，用于开发一项计划的第一阶段试点协议开发阶段，PI希望该阶段将导致开发和部署用于远程量化水生生物的原位生物传感器。该生物传感器将使用电化学检测来测量环境中的rDNA，而不需要通过聚合酶链式反应(PCR)进行靶向扩增。基于基因的遥感需要开发易于使用、快速、廉价、小型化的分析设备。DNA杂交的电化学检测是唯一有资格满足现场系统的大小、成本和功率要求的设备。这一第一阶段的开发将侧重于生物传感器技术开发的两个关键组成部分--远程提取DNA和不使用聚合酶链式反应扩增直接检测DNA。迎接这两个挑战是开发下一代遥感技术的关键第一步。特定物种数据的产生与相关环境变量的天气学测量相结合，可大大有助于理解与浮游生物动态、藻类水华的启动和珊瑚疾病传播有关的各种过程。监测有毒生物的生物传感器可以提供需要关闭渔业或海滩的早期警告。

Goodwin0332793ABSTRACTThe award provides funds to develop the Phase I Pilot - protocol development stage of a program that the PI's hope will lead to the development and deployment of an in-situ biosensor for remote quantification of aquatic organisms. The biosensor would use electrochemical detection to measure rDNA in the environment without target amplification by the polymerase chair reaction (PCR). Gene-based remote sensing requires the development of easy-to-use, fast, inexpensive, miniaturized analytical devices. Electrochemical detection of DNA hybridization is uniquely qualified to meet size, cost, and power requirements of field systems. This Phase I development will focus on two critical components of biosensor technology development - remote DNA extraction and direct detection of DNA without using PCR amplification. Meeting these two challenges are critical first steps toward developing the next generation of remote sensing technology. Species-specific data generation in conjunction with synoptic measurements of relevant environmental variables could significantly contribute to the understanding of a variety of processes related to plankton dynamics, initiation of algal blooms, and the spread of coral disease. Biosensors to monitor toxic organisms could provide early warnings of the need to close fisheries or beaches.****