Collaborative Research: Developing Gene-Based Remote Detection

该奖项提供资金用于开发项目的第一阶段试点协议开发阶段，PI希望该项目将开发和部署用于水生生物远程量化的原位生物传感器。该生物传感器将使用电化学检测来测量环境中的rDNA，而无需通过聚合酶椅反应（PCR）进行靶扩增。基于基因的遥感需要开发易于使用、快速、廉价、小型化的分析设备。电化学检测DNA杂交是唯一合格的，以满足尺寸，成本和功率要求的现场系统。第一阶段的开发将集中在生物传感器技术开发的两个关键组成部分——远程DNA提取和不使用PCR扩增的DNA直接检测。应对这两个挑战是发展下一代遥感技术的关键的第一步。特定物种数据的生成与相关环境变量的天气测量相结合，可大大有助于了解与浮游生物动态、藻华开始和珊瑚疾病蔓延有关的各种过程。监测有毒生物的生物传感器可以提供需要关闭渔场或海滩的早期预警。****

Goodwin0332793ABSTRACTThe award provides funds to develop the Phase I Pilot - protocol development stage of a program that the PI's hope will lead to the development and deployment of an in-situ biosensor for remote quantification of aquatic organisms. The biosensor would use electrochemical detection to measure rDNA in the environment without target amplification by the polymerase chair reaction (PCR). Gene-based remote sensing requires the development of easy-to-use, fast, inexpensive, miniaturized analytical devices. Electrochemical detection of DNA hybridization is uniquely qualified to meet size, cost, and power requirements of field systems. This Phase I development will focus on two critical components of biosensor technology development - remote DNA extraction and direct detection of DNA without using PCR amplification. Meeting these two challenges are critical first steps toward developing the next generation of remote sensing technology. Species-specific data generation in conjunction with synoptic measurements of relevant environmental variables could significantly contribute to the understanding of a variety of processes related to plankton dynamics, initiation of algal blooms, and the spread of coral disease. Biosensors to monitor toxic organisms could provide early warnings of the need to close fisheries or beaches.****