Collaborative Research: Developing Gene-Based Remote Detection

Goodwin0332793摘要该奖项提供资金用于开发第一阶段试点 - 项目的协议开发阶段，PI希望该项目将导致开发和部署用于远程量化水生生物的原位生物传感器。该生物传感器将使用电化学检测来测量环境中的 rDNA，而无需通过聚合酶椅反应 (PCR) 进行目标扩增。基于基因的遥感需要开发易于使用、快速、廉价、小型化的分析设备。 DNA 杂交的电化学检测具有独特的资格，可以满足现场系统的尺寸、成本和功率要求。这一阶段的开发将重点关注生物传感器技术开发的两个关键组成部分——远程 DNA 提取和不使用 PCR 扩增的直接 DNA 检测。应对这两个挑战是开发下一代遥感技术的关键的第一步。特定物种的数据生成与相关环境变量的天气测量相结合，可以极大地有助于理解与浮游生物动态、藻华爆发和珊瑚疾病传播相关的各种过程。监测有毒生物体的生物传感器可以提供需要关闭渔业或海滩的早期预警。****

Goodwin0332793ABSTRACTThe award provides funds to develop the Phase I Pilot - protocol development stage of a program that the PI's hope will lead to the development and deployment of an in-situ biosensor for remote quantification of aquatic organisms. The biosensor would use electrochemical detection to measure rDNA in the environment without target amplification by the polymerase chair reaction (PCR). Gene-based remote sensing requires the development of easy-to-use, fast, inexpensive, miniaturized analytical devices. Electrochemical detection of DNA hybridization is uniquely qualified to meet size, cost, and power requirements of field systems. This Phase I development will focus on two critical components of biosensor technology development - remote DNA extraction and direct detection of DNA without using PCR amplification. Meeting these two challenges are critical first steps toward developing the next generation of remote sensing technology. Species-specific data generation in conjunction with synoptic measurements of relevant environmental variables could significantly contribute to the understanding of a variety of processes related to plankton dynamics, initiation of algal blooms, and the spread of coral disease. Biosensors to monitor toxic organisms could provide early warnings of the need to close fisheries or beaches.****