Collaborative Research: Developing Gene-Based Remote Detection

Goodwin 0332793摘要该奖项提供资金，以发展第一阶段试点协议的发展阶段的计划，PI的希望将导致发展和部署的原位生物传感器的远程量化水生生物。生物传感器将使用电化学检测来测量环境中的rDNA，而不通过聚合酶椅式反应（PCR）进行靶扩增。基于基因的遥感需要开发易于使用、快速、廉价、小型化的分析设备。DNA杂交的电化学检测是唯一合格的，以满足现场系统的尺寸，成本和功率要求。第一阶段的开发将集中在生物传感器技术开发的两个关键组成部分-远程DNA提取和直接检测DNA，而不使用PCR扩增。迎接这两个挑战是开发下一代遥感技术的关键第一步。具体物种数据的生成与相关环境变量的天气测量相结合，可大大有助于了解与浮游生物动态、藻华爆发和珊瑚病蔓延有关的各种过程。监测有毒生物的生物传感器可以提供早期预警，说明需要关闭渔场或海滩。

Goodwin0332793ABSTRACTThe award provides funds to develop the Phase I Pilot - protocol development stage of a program that the PI's hope will lead to the development and deployment of an in-situ biosensor for remote quantification of aquatic organisms. The biosensor would use electrochemical detection to measure rDNA in the environment without target amplification by the polymerase chair reaction (PCR). Gene-based remote sensing requires the development of easy-to-use, fast, inexpensive, miniaturized analytical devices. Electrochemical detection of DNA hybridization is uniquely qualified to meet size, cost, and power requirements of field systems. This Phase I development will focus on two critical components of biosensor technology development - remote DNA extraction and direct detection of DNA without using PCR amplification. Meeting these two challenges are critical first steps toward developing the next generation of remote sensing technology. Species-specific data generation in conjunction with synoptic measurements of relevant environmental variables could significantly contribute to the understanding of a variety of processes related to plankton dynamics, initiation of algal blooms, and the spread of coral disease. Biosensors to monitor toxic organisms could provide early warnings of the need to close fisheries or beaches.****