WIDE-BORE 9.4 TESLA NMR SPECTROMETER FOR UW-MADISON

部分资金用于购买高分辨率、高视场、 (9.4\T），宽口径，多核磁共振谱仪的 生化磁共振设备将位于一楼， 生物化学系生物化学大楼的新翼， 威斯康星大学麦迪逊分校 仪器的选择 优化一维和二维NMR数据的收集和分析 套，并进行生物医学核磁共振实验，需要最终的 光谱仪灵敏度。 该仪器将使用约10%， 核研究的核磁共振设施，以开发新的方法， 大分子异核二维核磁共振波谱学，同位素 用于NMR分析的生物分子的标记，以及多核磁性 病毒、活细胞和完整组织的共振研究。 大约80% 光谱仪的时间将用于支持研究工作 十个主要的研究人员谁拥有美国国立卫生研究院同行评审的研究支持， 以下领域：蛋白质，核酸， 和天然产物;酶的活性部位和机制;酶的作用方式; 药物的作用;以及活细胞中代谢物的生理水平 和有机体。 剩下的10%的光谱仪时间将用于 培训和日常维护和维修。 日常管理 的仪器将由核磁共振设施的工作人员处理 在当地指导委员会的监督下， 主要用户群。 整个设施的整体性能 将由该领域专家咨询委员会定期审查 生物核磁共振

Partial funds are requested to purchase a high-resolution, high-field, (9.4\T), wide-bore, multinuclear magnetic resonance spectrometer for the Biochemical Magnetic Resonance Facility to be located in the first floor of the new wing of the Biochemistry Building, Department of Biochemistry, University of Wisconsin-Madison. The instrumentation has been chosen to optimize the collection and analysis of one- and two-dimensional NMR data sets and for carrying out biomedical NMR experiments requiring the ultimate in spectrometer sensitivity. The instrument is to be used about 10% for core research by the NMR facility to develop new approaches to hetero-nuclear two-dimensional NMR spectroscopy of macromolecules, isotopic labeling of biomolecules for NMR analysis, and multinuclear magnetic resonance studies of viruses, living cells, and intact tissues. About 80% of the spectrometer time will be devoted to supporting the research efforts of ten major investigators who have NIH peer-reviewed research support in the following areas: structure and function of proteins, nucleic acids, and natural products; active sites and mechanisms of enzymes; modes of action of drugs; and physiological levels of metabolites in living cells and organisms. The remaining 10% of the spectrometer time will be devoted to training and routine maintenance and repairs. Day-to-day administration of the instrumentation will be handled by the staff of the NMR facility with supervision of a Local Steering Committee including representatives from major user groups. The overall performance of the entire facility will be reviewed regularly by an Advisory Committee of experts in the field of biological NMR.