WIDE-BORE 9.4 TESLA NMR SPECTROMETER FOR UW-MADISON

要求部分资金购买高分辨率、高场强、 (9.4\T)，大口径多核磁共振波谱仪 生化核磁共振设施将设在 生物化学大楼的新翼，生物化学系， 威斯康星大学麦迪逊分校。该仪器已被选为 优化一维和二维核磁共振数据的收集和分析 用于进行生物医学核磁共振实验所需的 在分光计灵敏度方面。该仪器的使用率约为10% 核磁共振设施进行的核心研究，以开发新的方法来 同位素大分子的异核二维核磁共振波谱 用于核磁共振分析的生物分子的标记和多核磁性 对病毒、活细胞和完整组织的共振研究。约80% 的光谱仪时间将用于支持研究工作 在NIH获得同行评议研究支持的十位主要研究人员中 以下领域：蛋白质、核酸的结构和功能， 和天然产物；酶的活性部位和作用机理； 药物的作用；活细胞中代谢产物的生理水平 和有机体。剩下的10%的光谱仪时间将用于 培训以及日常维护和维修。日常管理 将由核磁共振设施的工作人员处理 在包括代表在内的地方指导委员会的监督下 来自主要用户群。整个设施的整体性能 将由外地专家咨询委员会定期审查 生物核磁共振仪。

Partial funds are requested to purchase a high-resolution, high-field, (9.4\T), wide-bore, multinuclear magnetic resonance spectrometer for the Biochemical Magnetic Resonance Facility to be located in the first floor of the new wing of the Biochemistry Building, Department of Biochemistry, University of Wisconsin-Madison. The instrumentation has been chosen to optimize the collection and analysis of one- and two-dimensional NMR data sets and for carrying out biomedical NMR experiments requiring the ultimate in spectrometer sensitivity. The instrument is to be used about 10% for core research by the NMR facility to develop new approaches to hetero-nuclear two-dimensional NMR spectroscopy of macromolecules, isotopic labeling of biomolecules for NMR analysis, and multinuclear magnetic resonance studies of viruses, living cells, and intact tissues. About 80% of the spectrometer time will be devoted to supporting the research efforts of ten major investigators who have NIH peer-reviewed research support in the following areas: structure and function of proteins, nucleic acids, and natural products; active sites and mechanisms of enzymes; modes of action of drugs; and physiological levels of metabolites in living cells and organisms. The remaining 10% of the spectrometer time will be devoted to training and routine maintenance and repairs. Day-to-day administration of the instrumentation will be handled by the staff of the NMR facility with supervision of a Local Steering Committee including representatives from major user groups. The overall performance of the entire facility will be reviewed regularly by an Advisory Committee of experts in the field of biological NMR.