Desorption Ionization Studies Using Fourier Transform Mass Spectrometry

该项目涉及分析和表面化学的一般领域，以及气相反应和质谱学的子领域。本研究的重点是发展生物大分子的结构表征方法，并利用傅里叶变换离子回旋共振(FT-ICR)研究离子捕获和离子检测的基本原理。本项目的重点将放在了解多肽在光解或与有机碱金属离子反应后的解离反应。对影响不同物种ICR信号的因素进行了具体研究，并利用FT-ICR对这些因素进行了分析。这项研究旨在更好地了解大的生物分子在吸收相对较高的能量光子或与碱金属离子发生反应后如何在气相中分解。本研究的最终目的是为高分子量生物分子的表征提供更好的能力。

This project is in the general area of analytical and surface chemistry and in the subfields of gas phase reactions and mass spectrometry. The focus of this research is to develop methods for structural characterization of large biomolecules and to study the fundamentals of ion trapping and ion detection using Fourier transform ion cyclotron resonance (FT-ICR). A large emphasis of this project will be placed on understanding dissociation reactions of peptides after photodissociation or reaction with organo-alkali metal ions. Specific studies are proposed to examine the factors which influence the ICR signal of various species which are analyzed using FT-ICR. This research is designed to better understand how large biomolecules will break apart in the gas phase after absortion of relatively high energy photons or reactions with alkali metal ion species. The ultimate goal of this research is to provide better capabilities for characterization of high molecular weight biomolecules.