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Desorption Ionization Studies Using Fourier Transform Mass Spectrometry

使用傅里叶变换质谱法进行解吸电离研究

基本信息

批准号：
9629966
负责人：
David Russell
金额：
$ 61.6万
依托单位：
Texas A&M Research Foundation
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
1996
资助国家：
美国
起止时间：
1996-07-01 至 2001-03-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9629966&HistoricalAwards=false
关键词：
Desorption Ionization Studies Using Fourier

项目摘要

This project, supported in the Analytical and Surface Chemistry Program, targets the construction and characterization of a tandem instrument using ion cyclotron resonance with ion mobility spectrometry and a time-of-flight mass spectrometer (ICR/IMS/TOF MS) for the determination of the volumes of large biological molecules. The ICR will select particular mass-to-charge ions, the IMS will sort these particular mass-to-charge ions by volume, and the TOF MS will detect these ions. Measurement of ion volumes for isomass ions will provide fractional composition information about different structural forms of these large biological molecules. In this work Professor Russell and his students of the Department of Chemistry at Texas A & M University will construct a new instrument that will be used in conjunction with matrix assisted laser desorption ionization (MALDI) MS to probe how phase parameters influence molecular conformation. The goal of this project is to build and characterize a new tandem mass spectrometer that can be used to determine the structure of large biological molecules. Professor Russell and his students at Texas A & M University will construct this instrument using principles of ion cyclotron resonance, an ion mobility spectrometer, and a time-of-flight mass spectrometer. This instrument will then be coupled to a matrix assisted laser desorption ionization source so that large biological molecules introduced into the gas phase from the solid matrix can be then sorted and characterized according to their structure. The aim is to determine the affect of the solid matrix upon the structure of the biological molecules. This new instrument will be useful in a number of areas in biotechnology and in the chemical sciences.

该项目在分析和表面化学计划的支持下，目标是使用离子回旋共振与离子迁移谱和飞行时间质谱仪（ICR/IMS/TOF MS）的串联仪器的构建和表征，用于测定大生物分子的体积。 ICR将选择特定的质荷比离子，IMS将按体积对这些特定的质荷比离子进行分类，TOF MS将检测这些离子。等质量离子的离子体积的测量将提供关于这些大生物分子的不同结构形式的分数组成信息。在这项工作中，罗素教授和他的学生在得克萨斯州A M大学化学系将构建一个新的仪器，将与基质辅助激光解吸电离（MALDI）MS结合使用，以探测相位参数如何影响分子构象。该项目的目标是建立和表征一种新的串联质谱仪，可用于确定大生物分子的结构。拉塞尔教授和他的学生在得克萨斯州A M大学将建造这个仪器使用离子回旋共振，离子迁移谱仪和飞行时间质谱仪的原则。然后将该仪器耦合到基质辅助激光解吸电离源，使得从固体基质引入气相的大生物分子然后可以根据其结构进行分选和表征。目的是确定固体基质对生物分子结构的影响。这种新仪器将在生物技术和化学科学的许多领域发挥作用。