Desorption Ionization Studies Using Fourier Transform Mass Spectrometry

使用傅里叶变换质谱法进行解吸电离研究

• 批准号: 9223629
• 负责人: David Russell
• 金额: $ 25.33万
• 依托单位: Texas A&M Research Foundation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-03-15 至 1996-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9223629&HistoricalAwards=false
• 关键词: Desorption; Ionization; Studies; Using; Fourier

This project is in the general area of analytical and surface chemistry and in the subfield of mass spectrometry. During the tenure of this three-year continuing grant, Professor Russell and his students will apply matrix assisted laser desorption ionization Fourier transform mass spectrometry (MALDI/FTMS) to the development of techniques to investigate the structure and reactivity of large biomolecules. This research, which builds upon results obtained under the aegis of NSF grant CHE-8821780, seeks to find a method for inducing sequence-specific cleavage of polypeptide and polynucleotide ions. A primary focus of Professor Russell's efforts centers around the use of excited state gas phase metal ions as a means to cause these large biomolecules of interest to fragment selectively. %%% The successful attainment of the goals of this research would provide a facile method for the rapid determination of structures of complex peptides and nucleic acids in the femtomole concentration regime. Such analytical methods are of extreme importance to advances in biotechnology and clinical chemistry.

该项目属于分析和表面化学的一般领域，以及质谱学的子领域。在这项为期三年的持续资助期间，Russell教授和他的学生将应用基质辅助激光解吸电离傅立叶变换质谱仪(MALDI/FTMS)来开发研究大生物分子的结构和反应性的技术。这项研究建立在美国国家科学基金会拨款CHE-8821780的支持下所获得的结果，试图找到一种诱导多肽和多核苷酸离子的序列特异性切割的方法。拉塞尔教授的主要工作重点是使用激发态气相金属离子作为一种手段，使这些感兴趣的大生物分子选择性地碎裂。本研究的成功实现将为在飞摩尔浓度范围内快速测定复杂多肽和核酸的结构提供一种简便的方法。这种分析方法对生物技术和临床化学的进步是极其重要的。