Posttranscriptional Regulation of Chloroplast Gene Expression

叶绿体基因表达的转录后调控

• 批准号: 8903578
• 负责人: Daniel Klessig
• 金额: $ 15万
• 依托单位: Rutgers University New Brunswick
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-08-15 至 1992-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8903578&HistoricalAwards=false
• 关键词: Posttranscriptional; Regulation; Chloroplast; Gene; Expression

This research is analyzing the light-mediated control of expression of the RuBPCase large subunit (LSU) gene at the post-transcriptional level. The primary emphasis involves molecular and biochemical analyses of chlorplast protein synthesis in cell-free extracts. The chloroplast transcriptioanl repressionof elongation which occurs in plants shifted into darkness is being studied both in vivo, to determine how ribosomal transit times vary, and in vitro, to characterize factor(s) responsible for the altered elongation rates in dark-shifted versus light-grown plants. Initial efforts involve the continued development of a chloroplast-derived, protein synthesizing extract that is programable with exogenously added mRNA. The chimeric LSU- CAT reporter gene will be employed in developing this assay. TAhe regulation of expression of genes coded by chloroplast DNA is a fundamental problem associated with understanding growth and development in higher plants. The research in this proposal is looking at the mechanisms of regulation of chloroplast gene expression through the development of a cell-free system that responds to the same factors found in the intact chloroplast. The development of such an assay will markedly enhance the ability of workers in this field to address issues related to the control of chloroplast protein synthesis.***//

本研究分析了光介导的 RuBPCase大亚基（LSU）基因在 转录后水平。 主要重点包括 叶绿体蛋白的分子和生化分析 在无细胞提取物中合成。 叶绿体 转录抑制的延伸，发生在 植物被转移到黑暗中正在进行研究， 以确定核糖体转运时间如何变化，在体外， 表征导致改变的因素 在暗移与光生长的植物中的伸长速率。 初步努力包括继续发展一个 叶绿体衍生的蛋白质合成提取物， 可编程的外源mRNA。 嵌合LSU- CAT报告基因将用于开发本试验。 叶绿体编码基因的表达调控 DNA是一个与理解有关的基本问题 高等植物的生长和发育。 的研究 该提案着眼于监管机制， 叶绿体基因的表达，通过发展的一个 一个无细胞系统，它对在 完整的叶绿体 这种分析的发展 将显著提高这一领域工作者的能力， 解决与控制叶绿体蛋白质有关的问题 * //