Molecular Genetic Analysis of det loci of Arabiodopsis thaliana

拟南芥基因座的分子遗传学分析

• 批准号: 8904106
• 负责人: Joanne Chory
• 金额: $ 33.68万
• 依托单位: The Salk Institute for Biological Studies
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-01 至 1993-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8904106&HistoricalAwards=false
• 关键词: Molecular; Genetic; Analysis; det; loci

The overall objective of this project is to elucidate the pathway of sensory perception associated with chloroplast development in plants. The process of greening, or differentiation of the chloroplast, involves the coordinate regulation of many nuclear and chloroplast genes. The cues for the initiation of this developmental program are both extrinsic (light) and intrinsic (cell-type and plastid signals). Several regulatory photoreceptor are involved in the perception of light signals, but nothing is known of the transduction elements that mediate either the external or intracellular signals. The proposed research program focusses on a novel class of Arabidopsis thaliana mutants previously isolated by the principal investigator. The mutant plants develop light-grown characteristics even when grown in darkness, including, leaf development and anthocyanin accumulation and are therefore designated det, for de-etionlated. There are at least 2 det complimentation groups. The specific strategic approach will be to characterize genetically, biochemically, and physiologically the previously identified det mutant, as well identify new det mutant classes. Double mutants will be made to begin analysis of gene interaction, which will eventually lead to construction of the path(s) which lead to greening. The characterization of these mutants has already suggested that the response to light may be a repression rather than an induction phenomenon. Further work promises to elucidate the greening pathways in plants, pathway which must be understood for practical as well as basic reason.

本项目的总体目标是阐明 与叶绿体相关的感官知觉途径 植物的发育。 绿化过程，或 叶绿体的分化，涉及协调 许多核和叶绿体基因的调控。 的线索 这一发展计划的启动， 外在（光）和内在（细胞类型和质体 信号）。 几种调节性光感受器参与 光信号的感知，但什么都不知道， 转导元件介导外部或 细胞内信号 拟议的研究计划 聚焦于一类新的拟南芥突变体 之前由主要研究者隔离。 的 突变植物即使在 在黑暗中生长，包括叶片发育和 花青素苷积累并因此被称为DET， 去离子 至少有两个det complement 组 具体的战略方针是 从基因、生化和生理上表征 先前鉴定DET突变体，以及鉴定新的 det突变类。 双变种人将开始 基因相互作用的分析，这将最终导致 建造通往绿化的道路。 这些突变体的特征已经表明 对光线的反应可能是一种压抑，而不是一种 感应现象 进一步的工作有望阐明 植物的绿化途径，必须了解的途径 因为实际的和基本的原因。