Molecular Genetic Analysis of det loci of Arabiodopsis thaliana

这个项目的总体目标是阐明与植物叶绿体发育相关的感觉感知途径。绿色化或叶绿体分化的过程涉及许多核基因和叶绿体基因的协调调控。启动这一发育程序的线索既有外在的(光)，也有内在的(细胞型和叶绿体信号)。一些调节性的光感受器参与光信号的感知，但对介导外部或细胞内信号的转导元件知之甚少。拟议的研究计划集中在一类新的拟南芥突变体上，这类突变体以前是由首席研究员分离的。这些突变植株即使在黑暗中生长也会表现出光生长的特征，包括叶片发育和花青素积累，因此被命名为DET。至少有两个DET恭维组。具体的战略方法将是从遗传、生化和生理上表征以前发现的Det突变体，以及确定新的Det突变体类别。双突变体将开始分析基因互作，这最终将导致路径的构建(S)，从而导致绿色。这些突变体的特征已经表明，对光的反应可能是一种抑制而不是诱导现象。进一步的工作有望阐明植物的绿化途径，这条途径必须被理解，因为实用和基本的原因。

The overall objective of this project is to elucidate the pathway of sensory perception associated with chloroplast development in plants. The process of greening, or differentiation of the chloroplast, involves the coordinate regulation of many nuclear and chloroplast genes. The cues for the initiation of this developmental program are both extrinsic (light) and intrinsic (cell-type and plastid signals). Several regulatory photoreceptor are involved in the perception of light signals, but nothing is known of the transduction elements that mediate either the external or intracellular signals. The proposed research program focusses on a novel class of Arabidopsis thaliana mutants previously isolated by the principal investigator. The mutant plants develop light-grown characteristics even when grown in darkness, including, leaf development and anthocyanin accumulation and are therefore designated det, for de-etionlated. There are at least 2 det complimentation groups. The specific strategic approach will be to characterize genetically, biochemically, and physiologically the previously identified det mutant, as well identify new det mutant classes. Double mutants will be made to begin analysis of gene interaction, which will eventually lead to construction of the path(s) which lead to greening. The characterization of these mutants has already suggested that the response to light may be a repression rather than an induction phenomenon. Further work promises to elucidate the greening pathways in plants, pathway which must be understood for practical as well as basic reason.