Brassinosteroid-auxin synergism to dissect hormone response pathways in Arabidopsis

油菜素类固醇-生长素协同作用剖析拟南芥中的激素反应途径

• 批准号: 0649389
• 负责人: Joanne Chory
• 金额: $ 72万
• 依托单位: The Salk Institute for Biological Studies
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0649389&HistoricalAwards=false
• 关键词: Brassinosteroid; auxin; synergism; dissect; hormone

A major unanswered question in developmental biology is how the final size of an animal or plant is determined. This study explores the roles of hormones in regulating organ size and stature in plants. Recent studies in the P.I.'s laboratory indicate that the plant hormones auxin and brassinosteroids (BRs) act in close proximity to regulate seedling growth. Using genetics, genomics, and physiological analyses, it was shown that the auxin and BR pathways are interconnected and this integration is likely to occur at the level of gene expression. This project will continue to explore the underlying molecular mechanism of this BR/auxin synergism and seek to identify new factors involved in growth regulation by BRs and auxin. These experiments will address the mechanism by which these hormones interact to regulate such diverse responses as changes in gene expression, cell expansion and division, and tissue differentiation. The long-term objective is to completely understand the signaling networks that regulate plant development in response to an ever-changing environment, and to relate this to strategies used in other organisms. An anticipated broader impact is a continuing commitment to post-graduate, graduate, and undergraduate education, as well as exposure of high school students to a research laboratory setting. Also, the computational analyses to be carried out allow access to publicly available datasets that are of general use to the molecular biology community, and we have made the information available through several recently implemented websites. Consequently, the analytical methods and the information generated by their implementation may be a valuable community resource.

发育生物学中一个尚未解决的主要问题是动物或植物的最终大小是如何确定的。本研究探讨了激素在调节植物器官大小和高度中的作用。 P.I.的最新研究的实验室表明，植物激素生长素和油菜素类固醇（BR）的作用非常接近，以调节幼苗的生长。 使用遗传学，基因组学和生理分析，它表明，生长素和BR途径是相互关联的，这种整合很可能发生在基因表达水平。本项目将继续探索BR/生长素协同作用的分子机制，并寻求识别参与BR和生长素生长调节的新因子。这些实验将解决这些激素相互作用的机制，以调节基因表达，细胞扩增和分裂以及组织分化的变化等不同的反应。长期目标是完全了解调节植物发育以应对不断变化的环境的信号网络，并将其与其他生物体中使用的策略联系起来。一个预期的更广泛的影响是继续致力于研究生，研究生和本科教育，以及高中学生接触到研究实验室设置。此外，要进行的计算分析允许访问公开的数据集，是一般使用的分子生物学界，我们已经通过几个最近实施的网站提供的信息。因此，分析方法及其实施所产生的信息可能是宝贵的社区资源。