Application of In Vivo Mapping Strategies to Order Arabidopsis YAC Libraries

应用体内作图策略订购拟南芥 YAC 文库

• 批准号: 9116923
• 负责人: Joanne Chory
• 金额: $ 44万
• 依托单位: The Salk Institute for Biological Studies
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-11-15 至 1996-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9116923&HistoricalAwards=false
• 关键词: Application; Vivo; Mapping; Strategies; Order

The objective of this award is the isolation, identification, and mapping of large segments of the Arabidopsis genome using yeast artificial chromosome (YAC) vectors and the construction of new Arabidopsis YAC libraries. This research takes advantage of newly developed pulse field gel sizing methods and yeast transformation approaches to enrich for Arabidopsis YAC clones that contain larger average inserts than are present in the existing libraries. The new, larger libraries should reduce the number of steps in a chromosome walking experiment by greater than a factor of two, thus facilitating the identification of specific genes of interest. The utility of the research lies in rapid distribution and dissemination of the library to the entire scientific community. Drs. Chory and Hoekstra will establish methods for clone archiving and data storage to catalog the library, and to place existing markers onto known clones in collaboration with the Howard Goodman's group at Massachusetts General Hospital. Copies of the YAC library will be distributed to interested researchers through the Arabidopsis Biological Resource Center at Ohio State University.

该奖项的目标是使用酵母人工染色体(YAC)载体分离、鉴定和定位拟南芥基因组的大片段，并构建新的拟南芥YAC文库。这项研究利用新开发的脉冲场凝胶大小方法和酵母转化方法来丰富包含比现有文库中存在的更大平均插入片段的拟南芥YAC克隆。新的、更大的文库应该会将染色体行走实验的步骤减少两倍以上，从而有助于识别感兴趣的特定基因。这项研究的用处在于向整个科学界迅速分发和传播图书馆。乔里博士和胡克斯特拉博士将建立克隆存档和数据存储的方法，为图书馆编目，并与马萨诸塞州总医院的霍华德·古德曼团队合作，将现有标记放置到已知克隆上。YAC文库的副本将通过俄亥俄州立大学的拟南芥生物资源中心分发给感兴趣的研究人员。