The Molecular Evolution of Prokaryotic DNA-Dependent RNA Polymerases: Research Fellowship in Marine Biotechnology

依赖DNA的RNA聚合酶是一种多亚基酶 对大多数生物体和古生物的存在至关重要 起源 这些性质使其成为一种理想的候选者， 分子计时器，既可以证实16S rRNA数据， 扩展了进化多样化的分析。 n这 奖学金研究，Palenik博士将使用抗体， 从鱼腥藻和E.杆菌 研究聚合酶亚基结构， “老”真细菌的序列（16S rRNA估计），如 Chloroflexus和各种蓝藻，prochlorophytes， 叶绿体 对获得的序列数据的分析将 提供了新的见解的进化发展， 光合生物 这项工作也可能导致 利用RNA聚合酶序列分析浮游植物 水生环境中的物种多样性。

DNA-dependent RNA polymerase is a multi-subunit enzyme critical to the existence of most organisms and of ancient origin. These properties make it an ideal candidate for a molecular chronometer, both to corroborate 16S rRNA data, and to extend the analysis of evolutionary diversification. n this fellowship research, Dr. Palenik will use antibodies to and oligonucleotide primers developed from Anabaena and E. coli polymerases to study the polymerase subunit structure and sequence of "older" eubacteria (16S rRNA estimate) such as Chloroflexus, and various cyanobacteria, prochlorophytes, and chloroplasts. Analysis of the sequence data obtained will provide new insights into the evolutionary development of photosynthetic organisms. The work will also likely result in the use of RNA polymerase sequences to analyze phytoplankton species diversity in aquatic environments.