Microbial Genome Sequencing: Life at the Land/Ocean Margin: Genomic Analysis of a Coastal Cyanobacterium

微生物基因组测序：陆地/海洋边缘的生命：沿海蓝细菌的基因组分析

• 批准号: 0333162
• 负责人: Brian Palenik
• 金额: --
• 依托单位: University of California-San Diego Scripps Inst of Oceanography
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-10-01 至 2007-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0333162&HistoricalAwards=false
• 关键词: Microbial; Genome; Sequencing; Life; Land

A grant has been awarded to Dr. Brian Palenik of the University of California-San Diego, Scripps Institution of Oceanography in collaboration with Dr. Ian Paulsen, The Institute for Genomic Research, to sequence and analyze the complete genome of a coastal marine cyanobacterium, Synechococcus sp. strain CC9311. This photosynthetic microorganism represents a widespread, ecologically relevant group that greatly contributes to the primary productivity of coastal environments. The investigators have hypothesized that this cyanobacterium, and coastal bacteria in general, have characteristic strategies for living in coastal, compared to open ocean, marine environments. For example these bacteria may have different or more protein pumps for excreting pollutants or toxic metals due to the higher level of these compounds in coastal environments. Using the genome sequence of CC9311 and comparing it to available genome sequences of open ocean cyanobacteria will help reveal these strategies.The information from this research will be available for future use to develop tools for monitoring cyanobacterial populations and their physiological status in coastal environments. Whether or not these microorganisms are being affected by pollutants at a particular coastal site can potentially be determined if genome information is available to develop monitoring techniques. Thus this project will contribute to the larger societal goal of monitoring coastal water quality. This project is also synergistic with costal marine projects funded by the NSF Microbial Observatories program. A broader impact of this project will be to communicate the basic concepts of genomics, their application to marine science, and the results of this research to the general public in the form of an exhibit at the Birch Aquarium, a significant tourist attraction and an important institution for science education in San Diego California. Training of future scientists (one graduate student and one post-doctoral fellow) will also occur in the emerging field of bioinformatics.

加州大学圣地亚哥分校斯克里普斯海洋学研究所的 Brian Palenik 博士与基因组研究所的 Ian Paulsen 博士合作获得了一笔资助，以对沿海海洋蓝细菌（聚球藻属）的完整基因组进行测序和分析。菌株CC9311。这种光合微生物代表了一个广泛分布的、与生态相关的群体，对沿海环境的初级生产力做出了巨大贡献。研究人员假设，与公海、海洋环境相比，这种蓝细菌以及一般的沿海细菌具有在沿海生活的特有策略。例如，由于沿海环境中这些化合物的含量较高，这些细菌可能具有不同或更多的蛋白质泵来排泄污染物或有毒金属。使用 CC9311 的基因组序列并将其与公海蓝藻的可用基因组序列进行比较将有助于揭示这些策略。这项研究的信息将供未来用于开发监测沿海环境中蓝藻种群及其生理状态的工具。如果基因组信息可用于开发监测技术，则可以确定这些微生物是否受到特定沿海地点污染物的影响。因此，该项目将有助于实现监测沿海水质的更大社会目标。该项目还与 NSF 微生物观测站计划资助的沿海海洋项目产生协同作用。该项目更广泛的影响将是通过在伯奇水族馆（加州圣地亚哥重要的旅游景点和重要的科学教育机构）举办展览的形式向公众传达基因组学的基本概念、基因组学在海洋科学中的应用以及这项研究的结果。未来科学家（一名研究生和一名博士后）的培训也将在新兴的生物信息学领域进行。