Molecular Biology of Cuticular Wax Biosynthesis

角质层蜡生物合成的分子生物学

• 批准号: 9017963
• 负责人: Patrick Schnable
• 金额: $ 28.1万
• 依托单位: Iowa State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-04-01 至 1994-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9017963&HistoricalAwards=false
• 关键词: Molecular; Biology; Cuticular; Wax; Biosynthesis

The composition of cuticular wax of plants is complex and incompletely known, and rather little is known of the biosynthesis of this material(s). Two of the seventeen loci known to be involved in biosynthesis of this wax in maize have been tagged with transposable elements; these genes will be cloned and, using the transposable element as a molecular probe, a study of the biosynthetic pathway (localization, structure, and regulation of the gene products) undertaken. Comparison of the events in maize with those of Arabidopsis is planned; these studies should lead to increased comprehension of the biochemical mechanisms involved in the synthesis of these unique and biologically significant plant lipids. The cuticular waxes of plants constitute the first barrier to the environment and the stresses it imposes on plants, and thus these waxes play an important role in plant survival. The composition of these waxes and the biosynthetic pathways by which they are formed are incompletely known. This project will make use of transposon-tagged cloned genes to examine the gene products of the biosynthetic pathway and thus enlarge our understanding of it.//

植物表皮蜡的组成复杂， 不完全知道，而知之甚少的生物合成 这些材料（S）。 在已知的17个基因座中， 参与玉米中这种蜡的生物合成的基因已经被标记为 转座因子;这些基因将被克隆， 转座因子作为分子探针， 生物合成途径（定位，结构和调控） 基因产品）。 玉米事件的比较 与拟南芥的那些计划;这些研究应该导致 增加对参与的生化机制的理解， 这些独特和具有生物学意义植物的合成 脂质。 植物的表皮蜡构成了植物的第一道屏障， 环境及其对植物的压力，因此这些 蜡在植物生存中起着重要作用。 所述组合物 这些蜡和它们的生物合成途径 形成的不完全了解。该项目将利用 转座子标记的克隆基因来检查基因产物 生物合成途径，从而扩大我们对它的理解。