High-density Genetic Map of Maize Transcripts

玉米转录本的高密度遗传图谱

• 批准号: 0321711
• 负责人: Patrick Schnable
• 金额: $ 369.13万
• 依托单位: Iowa State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-10-01 至 2007-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0321711&HistoricalAwards=false
• 关键词: density; Genetic; Map; Maize; Transcripts

To facilitate scientific advances and crop improvement, the thousands of maize genes being discovered during the maize genome sequencing project must be ordered relative to a genetic map. This project builds on prior NSF investments in maize genomics to genetically map 4,500 genes. The resulting high-density transcript map will enhance our understanding of the organization and evolution of the maize genome. These thousands of mapped genes will also provide the necessary sequence-based cross-links to facilitate the alignment of the rice physical map with the maize genetic map. This resource will provide a means to determine the linear order on maize chromosomes of maize genes. One thousand co-dominant genetic markers (Insertion Deletion Polymorphisms [IDP]s) suitable for use in a wide variety of genetic experiments will also be generated and distributed to the public.The research plan provides numerous, interdisciplinary training opportunities for a diverse group of undergraduate and graduate students at the interface between molecular and computational genomics. The project will use a variety of mechanisms to involve undergraduate students from a research university, an undergraduate college and underserved institutions. These trainees will be provided with intensive mentored research experiences. To help prepare them for scientific careers in a global environment, undergraduate students will visit to the International Maize and Wheat Improvement Center (Spanish acronym CIMMYT) in Mexico. Trainees will also be encouraged to return to the high schools from which they graduated to make short presentations regarding their research activities. Other outreach activities will include a program conducted in partnership with the Reiman Gardens at Iowa State University (http://www.reimangardens.iastate.edu) to expose elementary school students to plant biology.Deliverables:4,500 mapped genes and 1000 co-dominant genetic markers (Insertion Deletion Polymorphisms [IDP]s) will be generated and distributed to the public via the project database (http://pslab.agron.iastate.edu/research/genomics/htp_est/idps.shtml) as well as public databases such as PlantGDB (http://www.zmdb.iastate.edu/PlantGDB/), MaizeGDB (http://www.maizegdb.org/), and Gramene (http://www.gramene.org/).

为了促进科学进步和作物改良，在玉米基因组测序项目中发现的数千个玉米基因必须相对于遗传图谱进行排序。 该项目建立在NSF先前对玉米基因组学的投资基础上，对4,500个基因进行遗传图谱绘制。 由此产生的高密度转录图谱将增强我们对玉米基因组的组织和进化的理解。 这数千个绘制的基因还将提供必要的基于序列的交叉链接，以促进水稻物理图谱与玉米遗传图谱的比对。该资源将提供一种确定玉米基因在玉米染色体上的线性顺序的手段。 一千个共显性遗传标记（插入缺失多态性[IDP]）适用于各种遗传实验也将产生和分发给公众。研究计划提供了大量的，跨学科的培训机会，为不同群体的本科生和研究生在分子和计算基因组学之间的接口。 该项目将利用各种机制，让来自研究型大学、本科学院和服务不足机构的本科生参与。 这些受训人员将获得密集的指导研究经验。 为了帮助他们在全球环境中为科学事业做好准备，本科生将访问墨西哥的国际玉米和小麦改良中心（西班牙语缩写CIMMYT）。还将鼓励受训人员返回他们毕业的高中，就他们的研究活动作简短的介绍。 其他推广活动将包括与爱荷华州州立大学的雷曼花园合作开展的一项计划（http：www.reimangardens.iastate.edu），让小学生接触植物生物学。4，500个定位基因和1000个共显性遗传标记（插入/删除多态性[IDP]）将通过项目数据库生成并分发给公众（http：//pslab.agron.iastate.edu/research/genomics/htp_est/idps.shtml）以及公共数据库，如PlantGDB edu/PlantGDB/）、MaizeGDB（http：//www.maizegdb.org/）和Gramene（http：//www.gramene.org/）。