Molecular Biology of Cuticular Wax Biosynthesis

9316832 Schnable植物表皮蜡的组成是复杂的，并且不完全已知，关于这种材料的生物合成知之甚少。 glossy 8基因座是已知参与玉米中这种蜡生物合成的17个基因座之一，已通过用转座因子标记进行分子克隆。 此外，拟南芥的cer2基因座，这是参与表皮蜡的生物合成，已被定位到一个70 kb的基因组片段。 cer2基因座将通过遗传互补试验进行鉴定。 然后，这两个基因将被用来研究这些独特的和生物学意义的植物脂质的生物合成所涉及的生化和遗传机制。 植物的表皮蜡质是植物抵御外界环境和外界压力的第一道屏障。 因此，这些蜡在植物生存中发挥着重要作用。 这些蜡的组成和它们形成的生物合成途径还不完全清楚。 本计画将利用分子克隆的基因来研究生物合成途径的基因产物，进而扩大我们对生物合成途径的了解。 ***

9316832 Schnable The composition of the cuticular wax of plants is complex and incompletely known, and little is known regarding the biosynthesis of this material(s). The glossy8 locus, one of the seventeen loci known to be involved in the biosynthesis of this wax in maize, has been molecularly cloned by tagging with a transposable element. In addition, the cer2 locus of Arabidopsis, which is involved in cuticular wax biosynthesis, has been localized to a 70-kb segment of the genome. The cer2 locus will be identified by genetic complementation tests. Both genes will then be used to study the biochemical and genetic mechanisms involved in the biosynthesis of these unique and biologically significant plant lipids. The cuticular waxes of plants constitute the first barrier to the environment and the stresses it imposes on plants. Thus, these waxes lay an important role in plant survival. The composition of these waxes and the biosynthetic pathways by which they are formed are incompletely understood. This project will make use of molecularly cloned genes to examine the gene products of the biosynthetic pathway and thus enlarge our understanding of it. ***