Molecular Basis of Hydroid Allorecognition

这项研究的长期目标是识别，克隆， 序列基因编码同种识别决定簇， 殖民水螅，水螅。 迄今为止的工作表明， 产生在它们的特性上不同的同基因集落的可行性 组织相容性反应 在此期间， 申请人建议：1）继续进行分析， 在这个水螅体中的融合性的传递遗传学，2）产生， 在大量的情况下，来自同基因集落的组织， 融合性反应，和3）从这些产生cDNA文库 组织中 这一努力将产生必要的材料， 消减杂交作为分离基因的策略， 兴趣 众所周知，脊椎动物具有发达的免疫系统， 排斥外来组织的机制，但知之甚少 关于无脊椎动物的这种机制。 拟议研究 代表了一个无脊椎动物学家 和分子免疫学家 他们的最终目标是 第一次明确地描述了所涉及的分子 无脊椎动物的自我识别（组织型）反应。 的 这项研究的结果最终将解决长期以来， 无脊椎动物系统发育关系的悬而未决的问题 以及脊椎动物的自我识别和免疫样防御反应。

The long-range goal of this research is to identify, clone, and sequence genes encoding allorecognition determinants from the colonial hydroid, Hydractinia. Work to date has shown the feasibility of producing isogenic colonies which differ in their histocompatibility responses. During this grant period, the applicants propose to: 1) continue ongoing analysis of the transmission genetics of fusibility in this hydroid, 2) produce, in bulk, tissues from isogenic colonies which differ in their fusibility responses, and 3) generate cDNA libraries from these tissues. This effort will yield the materials necessary to pursue subtractive hybridization as a strategy for isolating the genes of interest. It is well known that vertebrate animals have well developed immune mechanisms for rejection of foreign tissues, but little is known about such mechanisms in invertebrates. The proposed research represents a collaborative effort between an invertebrate zoologist and a molecular immunologist. Their ultimate goal is to accomplish the first unambiguous characterization of the molecules involved in an invertebrate self-recognition (tissue-type) response. The results of this research ultimately should resolve the long- standing question of the phylogenetic relationships of invertebrate and vertebrate self-recognition and immune-like defense responses.