Insect Acid-Base Regulation

酸碱状态强烈影响许多关键的生理功能， 包括酶催化、离子平衡、膜转运， 受体结合。 我们对这一关键问题的理解 生理学方面是非常有限的。 的目的 本研究旨在建立细胞外（血）酸碱平衡模型 调节昆虫的行为，用蝗虫。 的作用 排泄和肾脏系统中的血液pH值调节将 哈里森博士检查;这两个系统主导的交换 与环境有关的酸碱化合物。 他将评估 肾系统利用自然（饥饿）和 人工酸胁迫 然后他会确定 酸碱调节位点 片段 这些实验将基于微电极， 滴定、生化和气体取样技术。 本研究 将产生第一个酸碱调节的综合模型 在昆虫中，并将提供对机制的一般见解 动物用来补偿生理压力的。

Acid-base status strongly effects many key physiological functions, including enzyme catalysis, ionic balance, membrane transport, and hormone-receptor binding. Our understanding of this critical aspect of physiology is extremely limited. The objective of this study is to develop a model for extracellular (blood) acid-base regulation in an insect, using the locust. The role of the ventilatory and renal system in blood pH regulation will be examined be Dr. Harrison; these two systems dominate exchange of acid-base relevant compounds with the environment. He will assess the role of the renal system using natural (starvation) and artificial acid stresses. Then he will determine the location of acid-base regulatory sites by cannulating individual renal segments. These experiments will be based on micro-electrode, titration, biochemical, and gas sampling techniques. This study will generate the first integrated model of acid-base regulation in an insect, and will provide general insight into the mechanisms used by animals to compensate for physiological stresses.