Cell Volume Regulation in Skate Erythrocytes

滑冰红细胞的细胞体积调节

• 批准号: 9102215
• 负责人: Leon Goldstein
• 金额: $ 54.7万
• 依托单位: Brown University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-12-01 至 1996-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9102215&HistoricalAwards=false
• 关键词: Cell; Volume; Regulation; Skate; Erythrocytes

Many animal and plant cells are exposed to environmental salinity changes which cause alterations in the normal volumes of these cells. These changes, if they remained uncorrected, would disturb or even destroy normal cell function. Therefore, cells have evolved mechanisms by which they can adjust their volumes following these disturbances. During the past decade we have been investigating the mechanisms of volume regulation in one type of cell. We found that the red blood cell (RBC) of the salinity-tolerant little skate is a good model in which to study cell volume regulation. During the upcoming grant period we plan to determine how transport proteins in the cell's membrane operate to reduce cell volume after volume expansion in a dilute medium. This will involve identifying the specific membrane proteins involved in the process and the "signaling" mechanisms that "tell" the proteins that cell volume is too large, thereby activating these proteins. The results obtained in this study will be important to our understanding of the basic principles of cell volume regulation. In addition they will assist in discovering the causes of disturbances, as well as development of new treatments for diseases affecting the body's ability to maintain normal cell volume.

许多动物和植物细胞暴露于环境盐度 这些变化会导致正常体积的改变， 细胞 这些变化，如果不加以纠正， 甚至破坏正常的细胞功能。 因此，细胞具有 进化出了一种机制， 这些骚乱。 在过去的十年里，我们 研究一种类型的 cell. 我们发现，耐盐的红细胞（RBC） Little Skate是研究细胞体积的良好模型 调控 在即将到来的赠款期间，我们计划确定 细胞膜中的转运蛋白如何减少 在稀释培养基中体积扩增后的细胞体积。 这将 涉及识别参与细胞膜的特异性膜蛋白， 过程和“信号”机制，“告诉”蛋白质 细胞体积太大，从而激活这些蛋白质。 这项研究所获得的结果将对我们的 了解细胞体积调节的基本原理。 此外，他们还将协助发现的原因， 干扰以及疾病新疗法的开发 影响身体维持正常细胞体积的能力。