Mechanisms of Anoxic Tolerance and Metabolic Depression

缺氧耐受和代谢抑制的机制

• 批准号: 8802045
• 负责人: Leon Goldstein
• 金额: $ 26.63万
• 依托单位: Brown University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-10-15 至 1992-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8802045&HistoricalAwards=false
• 关键词: Mechanisms; Anoxic; Tolerance; Metabolic; Depression

Dr. Jackson and his associates plan studies on mechanisms of acid-base, ionic, and metabolic regulation in reptiles and mammals. The reptile studies derive from past observations on the extraordinary anoxic tolerance of the freshwater turtle, Chrysemys picta bellii. Three directions will be pursued. First, the increase in plasma catecholamines (epinephrine and norepinephrine), that have been recenty observed when turtles are anoxic and/or acidotic, will be further documented and its functional significance and possible contribution to this animal's anoxic tolerance will be investigated. Second, the marked increase in plasma calcium concentration in turtles during anoxia leads us to a broad study of calcium kinetics and hormonal regulation of calcium metabolism in this species. He plans to compare the responses of the turtle to another reptile that lacks the turtle's mineral-rich shell. Third, because his recent results have failed to reveal a correlation between blood acidosis and anoxic tolerance or anaerobic metabolism he will investigate intracellular pH and metabolite control of selected tissues during various types of anoxic and acidotic conditions. The hypothesis presented in this project, that cellular acidosis depresses energy metabolism, relates to the final proposed project on the heterothermic bat, Eptesicus fuscus. Preliminary data suggest a dual pH control strategy in this species during torpor at 20 degrees centigrade: alphastat in the summer, pH- stat in the winter. The principal investigator's objective is to verify this observation in both blood and cells and evaluate its relevance to energy conservation of torpid bats. Overall, the goal is an understanding of the mechanisms regulating hypometabolic states.

杰克逊博士和他的同事计划研究 爬行动物的酸碱、离子和代谢调节， 哺乳动物 爬行动物的研究来源于过去对 淡水龟非凡的耐缺氧能力 红腹鼠 将朝着三个方向努力。 首先，血浆儿茶酚胺（肾上腺素和 去甲肾上腺素），这是最近观察到的，当海龟 缺氧和/或酸中毒，将进一步记录， 功能意义和可能的贡献 研究动物的缺氧耐受性。 二是 海龟血浆钙浓度显著增加， 缺氧导致我们对钙动力学和激素的广泛研究， 钙代谢的调节。 他计划 将海龟的反应与另一种缺乏 富含矿物质的龟壳 第三，因为他最近 结果未能揭示血液与 酸中毒和缺氧耐受或无氧代谢，他将 研究细胞内pH和代谢物控制 组织在各种类型的缺氧和酸中毒条件下。 在这个项目中提出的假设，即细胞酸中毒 抑制能量代谢，与最终提出的 一个关于异温蝙蝠的项目 初步 数据表明，在这一物种的双重pH控制策略， 在20摄氏度的麻木：alphastat在夏天，pH值- 在冬天的时候。 首席研究员的目标是 在血液和细胞中验证这一观察结果，并评估其 与蝙蝠的能量守恒有关。 总体看 目标是了解调节 低代谢状态