Regulation of Organic Osmolyte Channel Activity in Fish Erythrocytes

9974350Goldstein所有细胞都需要保持其体积恒定才能存活和正常发挥功能。然而，细胞体积的变化发生在细胞环境中发生的生理和病理压力期间。戈尔茨坦博士的研究建议旨在确定在环境干扰后，细胞膜上运行的使体积恢复正常的机制。他之前的工作表明，随着细胞体积的变化，细胞膜中一种名为带3的特定蛋白质被激活，以使红细胞中的细胞体积恢复正常。在人体内的大多数细胞中都发现了类似的蛋白质。在目前的研究方案中，他和他的同事们将确定带3是如何作为体积调节蛋白工作的，以及它是单独工作还是与细胞膜上的其他蛋白质一起工作来调节细胞体积。他们将利用现代细胞生理学和分子生物学技术来回答这些关键问题。另一个重要的问题是，细胞体积的变化如何激活细胞膜上的带3蛋白。在我们之前的研究中，他们已经部分确定了导致带3激活的生化途径。现在，他们计划使用一种高度复杂的新分析技术，确定带3分子的哪一部分在激活过程中发生变化，以及细胞内部的哪些酶影响这些变化。上述研究将对我们理解不仅是红细胞，而且是身体中的其他细胞，如脑细胞，如何应对环境中的中风等干扰做出重大贡献。希望通过这些研究和其他实验室的相关研究，能够开发出更好的治疗方法来治疗这些干扰。

9974350Goldstein All cells need to maintain their volume constant in order to survive and function normally. However, changes in cell volume take place during both physiological and pathological stresses occurring in the cell's environment. Dr. Goldstein's research proposal is directed at determining the mechanisms operating in the cells' membranes to return volume back to normal following the environmental disturbances.His previous work has suggested that following changes in cell volume a specific protein in the cell membrane, named band 3, is activated to restore cell volume back to normal in red blood cells. Similar proteins are found in most cells within the body. In the current research proposal he and his colleagues will determine how band 3 operates as a volume regulatory protein and whether it operates alone or in conjunction with other proteins in the cell membrane to regulate cell volume. They will employ modern cellular physiological and molecular biological techniques to answer these critical questions.Another important question, which they will investigate in this proposal, is how changes in cell volume activate the band 3 protein in the cell membrane. They have partially identified the biochemical pathway that causes band 3 activation during our previous studies. Now, using a highly sophisticated new analytical technique, they plan to identify what part of the band 3 molecule is changed during activation and which enzymes in the cell interior effect these changes.The research described above will make a significant contribution to our understanding of how not only red blood cells but also other cells in the body, such as brain cells, cope with disturbances such as stroke in their environment. Hopefully, better therapies will be able to be developed to treat these disturbances as a result of these studies and related ones from other laboratories.