A Sensor for Determining Viable Cell Density

活细胞密度是发酵过程中最重要的参数之一。过去已经介绍了许多间接测定细胞密度的方法。然而，这些方法缺乏特异性。提出了一种快速、明确地测量培养基中活细胞数密度的传感器。在该方法中，利用活细胞的特性将活细胞与其他惰性粒子区分开来。测量速度是通过快速计数来实现的。该传感器可用于单个培养而无需校准。将构建离线和在线传感器。

Viable cell density is one of the most important parameters in fermentation processes. Many indirect methods of determining cell density have been introduced in the past. However, these methods suffer for non-specificity. A sensor is proposed for rapid and explicit measurement of viable cell number density in culture media. In the proposed method, viable cells are distinguished from other inert particles by using the characteristics of living cells. The speed in measurement is achieved by fast counting. The sensor is usable in single cultures without calibration. Off-line as well as on-line sensors are to be constructed.