Engineering Research Equipment Grant: An Ultraviolet Exposure System

活菌密度的在线监测对于监测和控制发酵过程具有重要意义。提出了一种新的、在线的细胞密度监测仪，可以直接测量培养基中的活细胞密度。在这种方法中，活细胞通过利用活细胞的特征与其他惰性粒子或死亡细胞区分开来。通过快速计数实现了测量速度。该传感器可用于任何单细胞培养，无需校准。一种能够将传感器图像转移到硅片上的紫外线曝光系统将用于执行这一项目。

On-line monitoring of viable cell density is important in monitoring and controlling fermentation processes. A new, on-line cell density monitor is proposed which can measure viable cell density directly in a culture medium. In this method, viable cells are distinguished from other inert particles or dead cells by using the characteristics of the living cells. The speed in measurement is achieved by fast counting. The sensor will be usable in any single cell cultures without requiring calibrations. An ultraviolet exposure system capable of transferring a sensor image onto a silicon wafer is to be used to carry out this project.