Engineering Research Equipment Grant: Biosensor Laboratory

用于生物反应器的市售溶解氧探头 应用具有许多不期望的特性 校准漂移和流量灵敏度。 利用国家- 的最先进的集成电路制造技术，提出了 开发一种可蒸汽灭菌的溶解氧探头， 表现出这些缺点。 此外，具有感测尖端的微探针 将开发几微米的尺寸，用于监测当地 各种发酵底物和产物的浓度 固定化细胞层。 这些提出的传感器尤其 用于监测涉及剪切敏感哺乳动物的生物过程 细胞培养和固定化细胞，这获得了显着的 近年来，工业重要。 微电路制造技术成功地应用于 构建溶解氧探针有望打开广泛的 制造生物过程监测传感器的可能性 将军 通过在单个芯片上集成多个传感器， 可以同时监测参数。 通过加入酶 和单克隆抗体，可以制作传感器芯片进行检测 参与下游的各种小分子和大分子， 上游生物过程。 用于固定化细胞的微探针 应用程序将能够测量层。 等 测量，这在以前是不可能的， 预计将大大帮助科学家和工程师研究 固定化细胞的生理行为。 知识 然后可以将获得的结果应用于优化生物反应器操作 和设计。 因此，预计所提出的传感器将 在推进生物技术方面做出了重大贡献，这是一个 全国知名度。

Commercially available dissolved oxygen probes for bioreactor applications have a number of undesirable characteristics such as calibration drift and flow sensitivity. By making use of the state- of-the-art integrated circuit fabrication technology, it is proposed to develop a steam-sterilizable dissolved oxygen probe, which does not exhibit these shortcomings. Also, microprobes with a sensing tip of a few-micron size are to be developed for monitoring local concentrations of various fermentation substrates and products within the immobilized cell layers. These proposed sensors are especially useful for monitoring bioprocesses involving shear-sensitive mammalian cell cultures and immobilized cells, which gained significant industrial importance in recent years. A successful application of the microcircuit fabrication technology to constructing dissolved oxygen probes is expected to open wide ranging possibilities in fabricating sensors for bioprocess monitoring in general. By integrating several sensors on a single chip, many parameters can be monitored simultaneously. By incorporating enzymes and monoclonal antibodies, sensor chips can be made for detecting various small and large molecules involved in downstream as well as upstream bioprocesses. The proposed microprobes for immobilized cell applications will enable measurements of layers. Such measurements, which have not been possible previously, are expected to help scientists and engineers greatly in studying the physiological behaviors of the immobilized cells. The knowledge obtained can then be applied to optimitzing bioreactor operations and designs. The proposed sensors are thus expected to contribute significantly in advancing biotechnology, an area of national prominence.