Development of an Efficient Transformation-Regeneration System for Soybean (Glycine max)

在过去的十年中，对选定植物物种的转化-再生已经成为常规，并有助于通过基因工程生产改良的植物产品。然而，尽管付出了巨大的努力，但还没有为谷类和豆类这两种最重要的经济植物开发出有效的转化-再生系统。该项目以大豆为模型实验系统，为豆科作物开发这样一个系统进行了全面和系统的努力。研究人员是一名大豆组织培养和遗传学专家，也是一名细胞生物学专家，他们将通过识别参与再生的细胞来开发一种转化技术。如果成功，这样的技术将直接有助于豆类的基因工程，并间接有助于我们了解植物转化的基本机制。

During the past decade, the transformation-regeneration of selected plant species has become routine and contributed to the production of improved plant products through genetic engineering. However, despite a large effort, an efficient system for transformation- regeneration has not been developed for the two most economically important groups of plants, cereals and legumes. This project represents a comprehensive and systematic effort to develop such a system for legumes, using soybean as a model experimental system. The investigators, an expert on soybean tissue culture and genetics and an expert in cell biology, will develop a transformation technology by identifying the cells participating in regeneration. If successful, such a technology will contribute directly to the genetic engineering of legumes, and indirectly to our understanding of the basic mechanisms of plant transformation.