Upgrade of 11.7 T NMR spectrometer and NMR computer

这是一个升级宾夕法尼亚州立大学化学系现有核磁共振设施的项目。升级包括改进11.7T光谱仪的探测/频率产生系统，安装第三个通道用于三重共振三维数据集收集，并用Silicon Graphics工作站取代过时的MicroVAX II计算机。例如，升级后的仪器将用于研究DNA聚合酶I、T4聚合酶复合体、二氢叶酸还原酶和嘌呤生物合成新途径中的三种功能酶的催化和底物结合的因素；载脂蛋白的稳定性和折叠模式；测定蛋白质在溶液中的结构特征，以及蛋白质的一级结构引导蛋白质快速有效地折叠成天然构象的机制。

This is a project to upgrade the Nuclear Magnetic Resonance Facility existing within the Chemistry Department of Pennsylvania State University. The upgrade includes an improved detection/frequency generation system for the 11.7 T spectrometer, the installation of a third channel for triple-resonance three-dimensional data set collection, and the replacement of the obsolete MicroVAX II computer by Silicon Graphics workstations. The upgraded instrumentation will be used to study, for example, the factors underlying catalysis and substrate binding in DNA polymerase I, T4 polymerase complex, dihydrofolate reductase and trifunctional enzymes in the de novo pathway for purine biosynthesis; stability and folding patterns of apo b heme proteins; determination of structural features of proteins in solution; and the mechanism by which the primary structure of a protein directs the rapid and efficient folding to the native conformation.