Upgrade of 11.7 T NMR spectrometer and NMR computer

这是宾夕法尼亚州立大学化学系现有核磁共振设备的升级项目。升级包括改进11.7 T光谱仪的检测/频率生成系统，安装第三个通道用于三共振三维数据集收集，并用Silicon Graphics工作站替换过时的MicroVAX II计算机。升级后的仪器将用于研究嘌呤生物合成新途径中DNA聚合酶I、T4聚合酶复合物、二氢叶酸还原酶和三功能酶的催化和底物结合因素；载脂蛋白b血红素蛋白的稳定性和折叠模式溶液中蛋白质结构特征的测定以及蛋白质的初级结构如何将快速有效的折叠转化为天然构象的机制。

This is a project to upgrade the Nuclear Magnetic Resonance Facility existing within the Chemistry Department of Pennsylvania State University. The upgrade includes an improved detection/frequency generation system for the 11.7 T spectrometer, the installation of a third channel for triple-resonance three-dimensional data set collection, and the replacement of the obsolete MicroVAX II computer by Silicon Graphics workstations. The upgraded instrumentation will be used to study, for example, the factors underlying catalysis and substrate binding in DNA polymerase I, T4 polymerase complex, dihydrofolate reductase and trifunctional enzymes in the de novo pathway for purine biosynthesis; stability and folding patterns of apo b heme proteins; determination of structural features of proteins in solution; and the mechanism by which the primary structure of a protein directs the rapid and efficient folding to the native conformation.