Upgrade of 11.7 T NMR spectrometer and NMR computer

这是一个升级核磁共振的项目 化学系现有设施 宾夕法尼亚州立大学。 升级包括一个 改进的11.7探测/频率生成系统 T型光谱仪，安装第三通道， 三重共振三维数据集收集，以及 以下列方式取代过时的MicroVAX II电脑 Silicon Graphics工作站 升级后的仪器 将被用来研究，例如， DNA聚合酶I，T4中的催化和底物结合 聚合酶复合物，二氢叶酸还原酶和 嘌呤从头合成途径中的三功能酶 载脂蛋白B血红素生物合成、稳定性和折叠模式 蛋白质.蛋白质结构特性的测定 在溶液中;和机制，其中主要的 蛋白质的结构指导快速有效地 折叠成天然构象。

This is a project to upgrade the Nuclear Magnetic Resonance Facility existing within the Chemistry Department of Pennsylvania State University. The upgrade includes an improved detection/frequency generation system for the 11.7 T spectrometer, the installation of a third channel for triple-resonance three-dimensional data set collection, and the replacement of the obsolete MicroVAX II computer by Silicon Graphics workstations. The upgraded instrumentation will be used to study, for example, the factors underlying catalysis and substrate binding in DNA polymerase I, T4 polymerase complex, dihydrofolate reductase and trifunctional enzymes in the de novo pathway for purine biosynthesis; stability and folding patterns of apo b heme proteins; determination of structural features of proteins in solution; and the mechanism by which the primary structure of a protein directs the rapid and efficient folding to the native conformation.