Upgrade of 11.7 T NMR spectrometer and NMR computer

11.7T核磁共振波谱仪和核磁共振计算机升级

• 批准号: 9208210
• 负责人: Barbara Garrison
• 金额: $ 9.53万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-05-15 至 1995-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9208210&HistoricalAwards=false
• 关键词: Upgrade; 11.7; NMR; spectrometer; computer

This is a project to upgrade the Nuclear Magnetic Resonance Facility existing within the Chemistry Department of Pennsylvania State University. The upgrade includes an improved detection/frequency generation system for the 11.7 T spectrometer, the installation of a third channel for triple-resonance three-dimensional data set collection, and the replacement of the obsolete MicroVAX II computer by Silicon Graphics workstations. The upgraded instrumentation will be used to study, for example, the factors underlying catalysis and substrate binding in DNA polymerase I, T4 polymerase complex, dihydrofolate reductase and trifunctional enzymes in the de novo pathway for purine biosynthesis; stability and folding patterns of apo b heme proteins; determination of structural features of proteins in solution; and the mechanism by which the primary structure of a protein directs the rapid and efficient folding to the native conformation.

这是一个升级宾夕法尼亚州立大学化学系现有核磁共振设施的项目。 此次升级包括改进 11.7 T 光谱仪的检测/频率生成系统、安装用于三重共振三维数据集收集的第三个通道，以及用 Silicon Graphics 工作站替换过时的 MicroVAX II 计算机。 升级后的仪器将用于研究，例如，DNA聚合酶I、T4聚合酶复合物、二氢叶酸还原酶和嘌呤生物合成从头途径中的三功能酶的催化和底物结合的潜在因素； apo b 血红素蛋白的稳定性和折叠模式；测定溶液中蛋白质的结构特征；以及蛋白质一级结构引导快速有效折叠至天然构象的机制。