Molecular Cloning and Functional Analysis of p130, app60src associated protein

p130、app60src相关蛋白的分子克隆及功能分析

• 批准号: 9210188
• 负责人: Amy Bouton
• 金额: $ 1.8万
• 依托单位: University of Virginia Main Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-08-01 至 1995-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9210188&HistoricalAwards=false
• 关键词: Molecular; Cloning; Functional; Analysis; p130

This proposal is to study the interaction of a protein tyrosine kinase (pp60 src) and a putative substrate, p130. When naturally occuring point mutations occur in pp60 src, resulting in pp60 v- src, or when site-directed mutagenesis is performed, pp60 src becomes oncogenic. The pp60 src protein probably functions in cellular signal transduction as well as in cell transformation. The p130 substrate protein will be purified, peptides from it will be microsequenced, and a molecular clone encoding the protein will be isolated. Interactions between these two proteins and a third protein (p110) will be examined to try to determine their roles in src-mediated cell transformation and other cellular processes that pp60 src is involved in. The importance of the src homology 2 domain will be assessed; this domain is believed to be involved in protein-protein interactions related to signal transduction. %%% Cells contain a variety of enzymes (kinases) which can add phosphate residues to certain amino acids in susceptible proteins. Protein tyrosine kinases have been implicated in transformation of cells from normal growth to cancerous growth. The best studied of this group is pp60 src. This proposal involves the interaction of pp60 src with a putative substrate, p130, and will investigate the events following their complex formation which result in cell transformation and signal transduction.

本研究的目的是研究蛋白质酪氨酸 激酶（pp 60 src）和推定底物p130。当自然 发生点突变发生在pp 60 src中，导致pp 60 v- src，或者当进行定点诱变时，pp 60 src 变成致癌的。pp 60 src蛋白可能在 细胞信号转导以及细胞转化。的 p130底物蛋白将被纯化，来自它的肽将被纯化。 微测序，编码蛋白质的分子克隆将被 与世隔绝这两种蛋白质之间的相互作用 蛋白质（p110）将被检查，试图确定他们的作用， SRC介导的细胞转化和其它细胞过程 pp 60 src参与其中。src同源性的重要性2 域将被评估;该域被认为涉及 与信号转导相关的蛋白质-蛋白质相互作用。 %%% 细胞中含有多种酶（激酶）， 磷酸残基与易感蛋白质中的某些氨基酸结合。 蛋白质酪氨酸激酶已经被牵连在转化 从正常生长到癌变生长。最好的研究 该组是pp 60 src。这项建议涉及以下方面的相互作用： pp 60 src与假定的底物p130，并将研究 事件后，其复杂的形成，导致细胞 转化和信号转导。