Acquisition of a Zeiss CEM 902 Spectroscopic TEM to Replace a Zeiss EM 10A TEM.

兹建议购买一台新的电子显微镜，其中包括一台集成成像电子能谱仪，以取代1975年购买的旧显微镜。电子能谱仪可以在几种模式下使用。最简单的模式通过从成像路径中去除非弹性散射电子来提高图像的灵敏度。通过选择不同能量损失能级的电子，也可以改变图像的对比度。这种特性有用性的一个具体例子是植物或真菌细胞壁结构的检查。由于许多细胞壁成分与电子的相互作用不同，不同成分之间的对比可以通过选择特定的能量窗口来屏蔽电子来增强。利用电子能谱仪进行检测，改进了硼化抗体对组织切片的渗透。这台新显微镜将是植物科学显微镜设施唯一的电子显微镜。

This is a proposal to purchase a new electron microscope containing an integrated imaging electron energy spectrometer to replace an old microscope purchased in 1975. The electron energy spectrometer can be used in several modes. The simplest mode improves the acuity of images by removing inelastically scattered electrons from the imaging pathway. The contrast of images may also be changed by selecting electrons of different energy loss levels. A specific example of the usefulness of this feature is in the examination of plant or fungal cell wall structure. Because many cell wall components interact differently with electrons, the contrast between the different components can be enhanced by selecting particular energy windows to screen electrons. Improved penetration of label into tissue sections using boronated antibodies has been achieved using the electron energy spectrometer for detection. The new microscope will be the only electron microscope for the Plant Science Microscopy Facility.