Acquisition of a Zeiss CEM 902 Spectroscopic TEM to Replace a Zeiss EM 10A TEM.

购买 Zeiss CEM 902 光谱 TEM 来替换 Zeiss EM 10A TEM。

• 批准号: 9214419
• 负责人: Melvin Fuller
• 金额: $ 13.4万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-15 至 1995-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9214419&HistoricalAwards=false
• 关键词: Acquisition; Zeiss; CEM; 902; Spectroscopic

This is a proposal to purchase a new electron microscope containing an integrated imaging electron energy spectrometer to replace an old microscope purchased in 1975. The electron energy spectrometer can be used in several modes. The simplest mode improves the acuity of images by removing inelastically scattered electrons from the imaging pathway. The contrast of images may also be changed by selecting electrons of different energy loss levels. A specific example of the usefulness of this feature is in the examination of plant or fungal cell wall structure. Because many cell wall components interact differently with electrons, the contrast between the different components can be enhanced by selecting particular energy windows to screen electrons. Improved penetration of label into tissue sections using boronated antibodies has been achieved using the electron energy spectrometer for detection. The new microscope will be the only electron microscope for the Plant Science Microscopy Facility.

这是一份购买包含集成成像电子能谱仪的新电子显微镜的提案，以取代1975年购买的旧显微镜。电子能谱仪可以在几种模式下使用。最简单的模式通过从成像路径中去除非弹性散射的电子来提高图像的锐度。图像的对比度也可以通过选择不同能量损失水平的电子来改变。这一特征的实用性的一个具体例子是在植物或真菌细胞壁结构的检查中。由于许多细胞壁组件与电子的交互作用不同，因此可以通过选择特定的能量窗口来屏蔽电子来增强不同组件之间的对比度。通过使用电子能谱仪进行检测，利用硼化抗体改善了标记对组织切片的穿透性。新的显微镜将是植物科学显微镜设施的唯一电子显微镜。