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Enhanced Plasmid Stability Using Multiple Killer Genes

Enhanced Plasmid Stability Using Multiple Killer Genes
使用多个杀伤基因增强质粒稳定性
批准号：
9224864
负责人：
Thomas Wood
金额：
$4.73万
依托单位：
University of California-Irvine
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
1993
资助国家：
美国
项目状态：
已结题
起止时间：
1993-08-15 至 1996-07-31
来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9224864&HistoricalAwards=false
关键词：
EnhancedPlasmidStabilityUsingMultiple

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英文摘要
9224864 Wood This proposal is on research to investigate enhanced plasmid stability by using multiple killer genes. Enhanced plasmid stability can be useful for a higher level of expression of recombinant protien products in E. coli and for effective genetic engineering strategies for waste treatment and bioremediation. The two main points to be examined include: (1) the role of media composition and temperature on plasmid stability; and (2) the effect of using two killer genes for enhanced stability. Experiments in both chemostat and shake - flask cultures will be performed. In addition, some modeling will also be performed. ***
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