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Enhanced Plasmid Stability Using Multiple Killer Genes

Enhanced Plasmid Stability Using Multiple Killer Genes
使用多个杀伤基因增强质粒稳定性
批准号:
9224864
负责人:
Thomas Wood
金额:
$4.73万
依托单位国家:
美国
项目类别:
Standard Grant
财政年份:
1993
资助国家:
美国
项目状态:
已结题
起止时间:
1993-08-15 至 1996-07-31

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中文摘要
翻译
9224864 Wood该提案是关于通过使用多个杀伤基因来研究增强的质粒稳定性的研究。 提高质粒稳定性有助于重组蛋白在大肠杆菌中的高效表达。大肠杆菌和有效的基因工程策略,废物处理和生物修复。 要检查的两个要点包括:(1)培养基组成和温度对质粒稳定性的作用;和(2)使用两种杀伤基因增强稳定性的效果。 将在恒化器和摇瓶培养中进行实验。此外,还将进行一些建模。 ***
英文摘要
9224864 Wood This proposal is on research to investigate enhanced plasmid stability by using multiple killer genes. Enhanced plasmid stability can be useful for a higher level of expression of recombinant protien products in E. coli and for effective genetic engineering strategies for waste treatment and bioremediation. The two main points to be examined include: (1) the role of media composition and temperature on plasmid stability; and (2) the effect of using two killer genes for enhanced stability. Experiments in both chemostat and shake - flask cultures will be performed. In addition, some modeling will also be performed. ***
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