Enhanced Bioremediation Using Genetically-Engineered Microbes

拟议的研究重点是 使用基因工程 微生物有效地 净化有害废物 网站.的天然能力 细菌细胞降解有机物 化合物将得到加强， 基因技术 工程. 我们的目标是 改变基因信息 细胞中的基因 编码这些酶 用于降解 3-氯邻苯二酚将被解偶联 从野生型调节器中 机械. 通过更换 野生型启动子与一个 可以从外部控制， 细菌可以被改造成 降解柠檬酸类化合物 以更快的速度。 此外，本发明还提供了一种方法， 改变酶途径 能让细菌减少 最终残留浓度 地下的有毒废物 目前可以达到的水平。

The proposed research focuses on using genetically-engineered microorganisms to efficiently decontaminate hazardous waste sites. The natural ability of bacterial cells to degrade organic compounds will be enhanced using the techniques of genetic engineering. The goal will be to alter the genetic information of the cell such that the genes that code for the enzymes responsible for the degradation of 3-chlorocatechol will be uncoupled from their wild-type regulator machinery. By replacing the wild-type promoter with one that can be controlled externally, the bacteria can be engineered to degrade the recalcitrant compound at faster rates. In addition, modifying the enzymatic pathway will enable the bacteria to reduce the final residual concentration of toxic wastes in the soil below the level that is currently achievable.