2001 Technology for a Sustainable Environment: NSF/EPA Partnership: Metabolic Engineering of Monooxygenases for 1-Naphthol and Styrene Epoxide Formation (TSE01-D)

2001 年可持续环境技术：NSF/EPA 合作伙伴关系：1-萘酚和苯乙烯环氧化物形成的单加氧酶代谢工程 (TSE01-D)

• 批准号: 0611950
• 负责人: Thomas Wood
• 金额: $ 8.14万
• 依托单位: Texas A&M Engineering Experiment Station
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-10-15 至 2006-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0611950&HistoricalAwards=false
• 关键词: 2001; Technology; Sustainable; Environment; NSF

0124401WoodThe objective of this project is to optimize the activity and expression of monooxygenases for the purpose of converting naphthalene to 1-naphthol and (chiral) styrene epoxide. Replacing the current method for producing 1-naphthol and styrene epoxide with the proposed enzymatic alternative may prove to be more environmentally benign in that less solvent and by-product wastes will result. There are two components to the work: (1) enzyme "breeding" (University of Connecticut) and (2) regulation of expression and bioprocess optimization (University of Maryland), which are highly synergistic.

0124401Wood这个项目的目标是优化单加氧酶的活性和表达，以将萘转化为1-萘酚和(手性)环氧苯乙烷。用所提议的酶替代法替代目前生产1-萘酚和环氧苯乙烷的方法可能被证明是更环保的，因为将产生更少的溶剂和副产品废物。这项工作有两个组成部分：(1)酶“育种”(康涅狄格大学)和(2)表达调控和生物过程优化(马里兰大学)，它们是高度协同的。