课题基金 / 基金详情

2001 Technology for a Sustainable Environment: NSF/EPA Partnership: Metabolic Engineering of Monooxygenases for 1-Naphthol and Styrene Epoxide Formation (TSE01-D)

2001 Technology for a Sustainable Environment: NSF/EPA Partnership: Metabolic Engineering of Monooxygenases for 1-Naphthol and Styrene Epoxide Formation (TSE01-D)
2001 年可持续环境技术:NSF/EPA 合作伙伴关系:1-萘酚和苯乙烯环氧化物形成的单加氧酶代谢工程 (TSE01-D)
批准号:
0611950
负责人:
Thomas Wood
金额:
$8.14万
依托单位国家:
美国
项目类别:
Continuing Grant
财政年份:
2005
资助国家:
美国
项目状态:
已结题
起止时间:
2005-10-15 至 2006-02-28

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中文摘要
翻译
0124401Wood这个项目的目标是优化单加氧酶的活性和表达,以将萘转化为1-萘酚和(手性)环氧苯乙烷。用所提议的酶替代法替代目前生产1-萘酚和环氧苯乙烷的方法可能被证明是更环保的,因为将产生更少的溶剂和副产品废物。这项工作有两个组成部分:(1)酶“育种”(康涅狄格大学)和(2)表达调控和生物过程优化(马里兰大学),它们是高度协同的。
英文摘要
0124401WoodThe objective of this project is to optimize the activity and expression of monooxygenases for the purpose of converting naphthalene to 1-naphthol and (chiral) styrene epoxide. Replacing the current method for producing 1-naphthol and styrene epoxide with the proposed enzymatic alternative may prove to be more environmentally benign in that less solvent and by-product wastes will result. There are two components to the work: (1) enzyme "breeding" (University of Connecticut) and (2) regulation of expression and bioprocess optimization (University of Maryland), which are highly synergistic.
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