Directed Evolution of Aromatic Dioxygenases for Trinitrotoluene and Aminodinitrotoluene Degradation
用于三硝基甲苯和氨基二硝基甲苯降解的芳香族双加氧酶的定向进化
基本信息
- 批准号:0606195
- 负责人:
- 金额:$ 9.46万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2005
- 资助国家:美国
- 起止时间:2005-10-15 至 2006-06-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
0114126 Smets The objective of this research project is to use an innovative protein engineering approach to create dioxygenases that initiate aerobic attack on trinitrotoluene (TNT) and aminodinitrotoluenes (ADNT), products of natural attenuation of TNT. The approach will be to expand the substrate range of nitroarene dioxygenases to allow oxidative transformation of ADNT and/or TNT. The gene encoding the large subunit of the terminal oxygenase will be the focus of this work. DNA shuffling will be used to create variants of two 2,4-dinitrotoluene (DNT) dioxygenases individually. Burkholdria sp. strain DNT will be used as the initial host to create a complete pathway for TNT/ADNT degradation. Creation of a complete, successful ADNT and/or TNT mineralization pathway will be initiated. Hence, the enzymatic steps for the down-stream processing of expected transformation products will also be addressed. Using a novel, directed-evolution approach and metabolic engineering, this project aims to create the first oxidative enzyme and pathway for the biomineralization of TNT/ADNT. The constructed strain will be invaluable in developing sustainable strategies for remediation of explosives contaminated environments. ***
0114126 Smets该研究项目的目的是使用创新的蛋白质工程方法来创建双加氧酶,启动对三硝基甲苯(TNT)和氨基二硝基甲苯(ADNT)的有氧攻击,TNT的天然衰减产物。 该方法将扩大硝基芳烃双加氧酶的底物范围,以允许ADNT和/或TNT的氧化转化。 编码末端加氧酶大亚基的基因将是这项工作的重点。 DNA改组将被用来创建两个2,4-二硝基甲苯(DNT)双加氧酶单独的变体。 伯克霍尔德氏菌属菌株DNT将用作初始宿主,以创建TNT/ADNT降解的完整途径。 将开始创建完整、成功的ADNT和/或TNT矿化途径。 因此,还将讨论预期转化产物下游加工的酶促步骤。 利用一种新的定向进化方法和代谢工程,该项目旨在创建第一个用于TNT/ADNT生物矿化的氧化酶和途径。 构建的菌株将是非常宝贵的,在制定可持续的战略,修复爆炸物污染的环境。***
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Thomas Wood其他文献
Neuroprotection after infection-sensitized neonatal hypoxic-ischemic brain injury
- DOI:
10.1186/2194-7791-2-s1-a11 - 发表时间:
2015-07-01 - 期刊:
- 影响因子:3.400
- 作者:
Hemmen Sabir;Damjan Osredkar;Elke Maes;Thomas Wood;Marianne Thoresen - 通讯作者:
Marianne Thoresen
A Systematic Review of Rehabilitation Protocols after Surgical Repair of the Extensor Tendons in Zones V–VIII of the Hand
- DOI:
10.1016/j.jht.2011.06.005 - 发表时间:
2011-10-01 - 期刊:
- 影响因子:
- 作者:
Mojib Sameem;Thomas Wood;Teegan Ignacy;Achilleas Thoma;Nick Strumas - 通讯作者:
Nick Strumas
A Trusted Mechanised Specification of JavaScript: One Year On
值得信赖的 JavaScript 机械化规范:一年后
- DOI:
- 发表时间:
2015 - 期刊:
- 影响因子:0
- 作者:
Philippa Gardner;Gareth Smith;Conrad Watt;Thomas Wood - 通讯作者:
Thomas Wood
Femtosecond-laser-induced creation of G and W color centers in silicon-on-insulator substrates
飞秒激光诱导在绝缘体上硅基板中产生 G 和 W 色心
- DOI:
- 发表时间:
2024 - 期刊:
- 影响因子:4.6
- 作者:
Hugo Quard;Mario Khoury;Andong Wang;T. Herzig;Jan Meijer;S. Pezzagna;S. Cueff;David Grojo;M. Abbarchi;Hai Son Nguyen;Nicolas Chauvin;Thomas Wood - 通讯作者:
Thomas Wood
Megalourethra associated with normal corpus spongiosum and dorsal chordee
- DOI:
10.1016/0090-4295(89)90323-3 - 发表时间:
1989-11-01 - 期刊:
- 影响因子:
- 作者:
Thomas Wood;Brent W. Snow - 通讯作者:
Brent W. Snow
Thomas Wood的其他文献
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{{ truncateString('Thomas Wood', 18)}}的其他基金
SeptProtect: Rapid effector discovery to protect wheat from Septoria tritici blotch disease
SeptProtect:快速发现效应子以保护小麦免受小麦斑枯病的侵害
- 批准号:
BB/X01617X/1 - 财政年份:2024
- 资助金额:
$ 9.46万 - 项目类别:
Research Grant
BBSRC Institute Strategic Programme: Building Robustness in Crops (BRIC) Partner Grant
BBSRC 研究所战略计划:增强作物稳健性(金砖四国)合作伙伴赠款
- 批准号:
BB/Y003039/1 - 财政年份:2023
- 资助金额:
$ 9.46万 - 项目类别:
Research Grant
EAGLE: Enhanced Analytical and Genetics Tools for Improving UK Food Legumes
EAGLE:增强的分析和遗传学工具,用于改善英国食品豆类
- 批准号:
BB/W019221/1 - 财政年份:2023
- 资助金额:
$ 9.46万 - 项目类别:
Research Grant
Pulse-Downy Mildew Pathosystem: deploying disease resistance, pathogenomics and microbial biocontrol
脉冲霜霉病病理系统:部署抗病性、病原学和微生物生物防治
- 批准号:
BB/T016019/1 - 财政年份:2020
- 资助金额:
$ 9.46万 - 项目类别:
Research Grant
PANACEA: Pathogenomics for enhancing food security in East Africa
PAACEA:加强东非粮食安全的病理基因组学
- 批准号:
BB/R015376/1 - 财政年份:2018
- 资助金额:
$ 9.46万 - 项目类别:
Research Grant
Collaborative Research: A Systems Biology Approach for Metabolically Engineering Escherichia coli for Producing Hydrogen via Fermentation
合作研究:通过代谢工程大肠杆菌发酵生产氢气的系统生物学方法
- 批准号:
1212320 - 财政年份:2012
- 资助金额:
$ 9.46万 - 项目类别:
Standard Grant
Collaborative Research: A Systems Biology Approach for Metabolically Engineering Escherichia coli for Producing Hydrogen via Fermentation
合作研究:通过代谢工程大肠杆菌发酵生产氢气的系统生物学方法
- 批准号:
0753702 - 财政年份:2008
- 资助金额:
$ 9.46万 - 项目类别:
Standard Grant
2001 Technology for a Sustainable Environment: NSF/EPA Partnership: Metabolic Engineering of Monooxygenases for 1-Naphthol and Styrene Epoxide Formation (TSE01-D)
2001 年可持续环境技术:NSF/EPA 合作伙伴关系:1-萘酚和苯乙烯环氧化物形成的单加氧酶代谢工程 (TSE01-D)
- 批准号:
0611950 - 财政年份:2005
- 资助金额:
$ 9.46万 - 项目类别:
Continuing Grant
Collaborative Research: Mating Signals and Demographic Divergence: Species Comparisons and Experimental Tests
合作研究:交配信号和人口差异:物种比较和实验测试
- 批准号:
0132502 - 财政年份:2002
- 资助金额:
$ 9.46万 - 项目类别:
Standard Grant
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Galaxy Analytical Modeling
Evolution (GAME) and cosmological
hydrodynamic simulations.
- 批准号:
- 批准年份:2025
- 资助金额:10.0 万元
- 项目类别:省市级项目
Understanding structural evolution of galaxies with machine learning
- 批准号:n/a
- 批准年份:2022
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- 批准号:10903001
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Tracing the evolution of aromatic molecules from interstellar space to planetary systems
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