Instrumentation for the Enhancement of Molecular Biology Program

增强分子生物学项目的仪器

• 批准号: 9251762
• 负责人: Robert Meyer
• 金额: $ 0.63万
• 依托单位: Chestnut Hill College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-05-15 至 1994-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9251762&HistoricalAwards=false
• 关键词: Instrumentation; Enhancement; Molecular; Biology; Program

The aim of the project is the enhancement of the recombinant DNA lab experience for undergraduate women in the biology department. Funds are requested for the purchase of four instrumentation units to meet the needs of the development plan: (1) A DNA thermal cycler will be used to select and amplify a specific eukaryotic gene from a eukaryotic cDNA library by polymerase chain reaction (PCR). (2) A minifluorimeter will be utilized for accurate DNA concentration determinations. (3) An electroporation system will allow efficient transformation of bacteria with cloned DNA in an expression vector. (4) A mini electrophoresis system will be used to perform electrophoresis of proteins and subsequent Western blotting to detect specific proteins immunologically.

该项目的目的是增强重组 DNA实验室经验为本科妇女在生物学 部门 请拨资金用于购买四架 仪器仪表单位，以满足发展的需要 计划：（1）DNA热循环仪将用于选择和 从真核cDNA中扩增出特定的真核基因 通过聚合酶链反应（PCR）扩增文库。 (2)一 微型荧光计将用于精确的DNA浓度 决心。 (3)电穿孔系统将允许 用克隆的DNA有效地转化细菌 表达载体 (4)一个微型电泳系统将 用于进行蛋白质电泳和随后的 免疫印迹法检测特异性蛋白质。