US-Polish Research on Chemistry of Phosphorus Selenium Compounds

亚利桑那州图森大学的理查德·S·格拉斯博士和简·米哈尔斯基博士共同开展了这项关于磷硒化合物化学的美国-波兰联合合作研究，和波兰科学院分子和大分子研究中心的亚历山大·斯科夫朗斯卡。研究人员将发展硒磷酸盐的化学，设计化学选择性合成有机硒化合物，作为将硒部分选择性引入蛋白质和RNA中的模型，并进行鉴定，与正品相比，酶法生产的P-Se化合物。拟议的国际合作是互惠互利的，因为波兰集团在困难的有机磷-硒化合物合成方面的广泛经验和专业知识得到了补充美国集团的机械论和77核磁共振波谱经验。这一合作将加强和扩大合作小组的研究努力和基础设施。所提出的将硒引入生物分子的化学选择性方法，如果成功，将允许引入有价值的77Se核磁共振光谱标记。这一标签将允许使用核磁共振波谱对这些大分子进行结构研究(美国研究小组和美国国立卫生研究院的T.C.Stadtman博士在该项目中合作)。这种方法还将有助于合成活性部位为硒的半合成酶，以及合成生物分子的衍生物。用于多波长反常衍射结构研究。这项拟议的化学研究通过使美国和波兰的顶尖专家能够将互补的人才结合在一起，并在相互感兴趣和能力很强的领域汇集研究资源，从而实现了推进科学的计划目标。

This U.S.-Poland Joint Cooperative Research on Chemistry of Phosphorous Selenium Compounds is between Dr. Richard S. Glass of the University of Arizona Tucson and Drs. Jan Michalski, and Aleksandra Skowronska of the Centre of Molecular and Macromolecular Studies of the Polish Academy of Studies. The researchers will develop the chemistry of selenophosphates, devise chemoselective synthesis of organoselenium compounds as models for selective introduction of selenium moieties into proteins and RNA's, and identification, by comparison with authentic compounds, of P-Se compounds produced enzymatically. The proposed international collaboration is mutually beneficial because the Polish group's extensive experience and expertise in the difficult synthesis of organophosphorus-selenium compounds is complemented by the mechanistic and 77 NMR spectroscopic experience of the American group. This collaboration will strengthen and broaden the research efforts and infrastructure of the collaborating groups. The proposed chemoselective methods for introducing selenium into biomolecules, if successful, would permit introduction of the valuable 77Se NMR spectroscopic label. This label would allow structure studies using NMR spectroscopy of these macromolecules (a project in which the American group and Dr. T.C. Stadtman of NIH collaborate). Such methodology would also facilitate synthesis of semisynthetic enzymes with selenium at their active site with potentially new and useful catalytic activities as well as synthesis of derivatives of biomolecules for multiwavelength anomalous diffraction structural studies. This proposed research in chemistry fulfills the program objectives of advancing science by enabling leading experts in the U.S. and the Poland to combine complementary talents and pool research resources in areas of strong mutual interest and competence.